EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.132 | expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.132 | Fe2+ | 1 mM, 60% inhibition | Bacillus sp. (in: Bacteria) | |
3.2.1.132 | Hg2+ | 1 mM, 44% inhibition | Bacillus sp. (in: Bacteria) |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.132 | extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.132 | Ca2+ | 1 mM, 20% activation | Bacillus sp. (in: Bacteria) | |
3.2.1.132 | Mn2+ | 1 mM, 37% activation | Bacillus sp. (in: Bacteria) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 52000 | - |
x * 52000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.132 | additional information | Bacillus sp. (in: Bacteria) | the enzyme is constitutively produced | ? | - |
? | |
3.2.1.132 | additional information | Bacillus sp. (in: Bacteria) MET 1299 | the enzyme is constitutively produced | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.132 | Bacillus sp. (in: Bacteria) | - |
- |
- |
3.2.1.132 | Bacillus sp. (in: Bacteria) MET 1299 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.132 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 476 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.132 | chitosan + H2O | high activity on 90% deacetylated colloidal chitosan | Bacillus sp. (in: Bacteria) | chitohexaose + chitopentaose + chitotetraose + chitotriose + chitobiose | chitosan is hydrolyzed to chitohexaose and chitopentaose at the initial stage of the reaction, after incubation for 1 h, the amounts of chitobiose, chitotriose and chitotetraose in the hydrolysate is increased | ? | |
3.2.1.132 | chitosan + H2O | high activity on 90% deacetylated colloidal chitosan | Bacillus sp. (in: Bacteria) MET 1299 | chitohexaose + chitopentaose + chitotetraose + chitotriose + chitobiose | chitosan is hydrolyzed to chitohexaose and chitopentaose at the initial stage of the reaction, after incubation for 1 h, the amounts of chitobiose, chitotriose and chitotetraose in the hydrolysate is increased | ? | |
3.2.1.132 | additional information | the enzyme is constitutively produced | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.2.1.132 | additional information | no activity on colloidal chitin | Bacillus sp. (in: Bacteria) | ? | - |
? | |
3.2.1.132 | additional information | the enzyme is constitutively produced | Bacillus sp. (in: Bacteria) MET 1299 | ? | - |
? | |
3.2.1.132 | additional information | no activity on colloidal chitin | Bacillus sp. (in: Bacteria) MET 1299 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.132 | ? | x * 52000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 60 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 40 | 70 | 40°C: about 40% of maximal activity, 70°C: about 15% of maximal activity | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 60 | - |
pH 5.5-9.0, stable below | Bacillus sp. (in: Bacteria) |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 5.5 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 4 | 6 | pH 4.0: about 45% of maximal activity, pH 6.0: about 25% of maximal activity | Bacillus sp. (in: Bacteria) |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.132 | 5.5 | 9 | stable at temperatures below 60°C | Bacillus sp. (in: Bacteria) |