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Literature summary extracted from

  • Kang, H.K.; Lee, J.H.; Kim, D.; Day, D.F.; Robyt, J.F.; Park, K.H.; Moon, T.W.
    Cloning and expression of Lipomyces starkeyi alpha-amylase in Escherichia coli and determination of some of its properties (2004), FEMS Microbiol. Lett., 233, 53-64.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 gene LSA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli Lipomyces starkeyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 acarbose
-
 Lipomyces starkeyi
3.2.1.1 Cu2+
-
 Lipomyces starkeyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+ stimulates Lipomyces starkeyi
3.2.1.1 Mg2+ stimulates Lipomyces starkeyi
3.2.1.1 additional information no effect by 1 mM EGTA Lipomyces starkeyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 68709
-
 x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE Lipomyces starkeyi
3.2.1.1 73000
-
 x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE Lipomyces starkeyi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Lipomyces starkeyi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 maltodextrin G5 + H2O
-
 Lipomyces starkeyi maltodextrin G2 + maltodextrin G3
-
 ?
3.2.1.1 maltodextrin G6 + H2O
-
 Lipomyces starkeyi maltodextrin G2 + maltodextrin G4
-
 ?
3.2.1.1 maltodextrin G6 + H2O
-
 Lipomyces starkeyi 2 maltodextrin G3
-
 ?
3.2.1.1 maltodextrin G7 + H2O
-
 Lipomyces starkeyi maltodextrin G3 + maltodextrin G4
-
 ?
3.2.1.1 additional information no activity with maltodextrins of G2-G4 Lipomyces starkeyi ?
-
 ?
3.2.1.1 starch + H2O soluble starch Lipomyces starkeyi malto-oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE Lipomyces starkeyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 40
-
-
 Lipomyces starkeyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6
-
-
 Lipomyces starkeyi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5 8 16 h, purified recombinant His-tagged enzyme, 90% stable Lipomyces starkeyi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.1 0.0034
-
 acarbose pH 6.0, 40°C, recombinant His-tagged enzyme Lipomyces starkeyi