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Literature summary extracted from

  • Braaz, R.; Handrick, R.; Jendrossek, D.
    Identification and characterisation of the catalytic triad of the alkaliphilic thermotolerant PHA depolymerase PhaZ7 of Paucimonas lemoignei (2003), FEMS Microbiol. Lett., 224, 107-112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 expression in Bacillus subtilis Paucimonas lemoignei

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.75 A134G PHB depolymerase activity similar to wild type Paucimonas lemoignei
3.1.1.75 A134G, H135L PHB depolymerase activity similar to wild type, 2fold lipase activity compared to wild type Paucimonas lemoignei
3.1.1.75 D242A no PHB depolymerase activity Paucimonas lemoignei
3.1.1.75 D242N no PHB depolymerase activity Paucimonas lemoignei
3.1.1.75 D256A PHB depolymerase activity similar to wild type Paucimonas lemoignei
3.1.1.75 D256N PHB depolymerase activity similar to wild type Paucimonas lemoignei
3.1.1.75 H306A no PHB depolymerase activity Paucimonas lemoignei
3.1.1.75 H47A no PHB depolymerase activity Paucimonas lemoignei
3.1.1.75 S136A no PHB depolymerase activity Paucimonas lemoignei
3.1.1.75 S136C no expression Paucimonas lemoignei
3.1.1.75 S136T 20% PHB depolymerase activity Paucimonas lemoignei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Paucimonas lemoignei
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Paucimonas lemoignei specific for amorphous PHB poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Paucimonas lemoignei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75
-
 Paucimonas lemoignei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 culture fluid
-
 Paucimonas lemoignei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 4-nitrophenyl butanoate + H2O wild type lipase activity is rather low Paucimonas lemoignei 4-nitrophenol + butanoate
-
 ?
3.1.1.75 4-nitrophenyl hexanoate + H2O wild type lipase activity is rather low Paucimonas lemoignei 4-nitrophenol + hexanoate
-
 ?
3.1.1.75 4-nitrophenyl octanoate + H2O wild type lipase activity is rather low Paucimonas lemoignei 4-nitrophenol + octanoate
-
 ?
3.1.1.75 p-nitrophenyl acetate + H2O
-
 Paucimonas lemoignei p-nitrophenol + acetate
-
 ?
3.1.1.75 p-nitrophenyl-decanoate + H2O wild type lipase activity is rather low Paucimonas lemoignei p-nitrophenol + decanoate
-
 ?
3.1.1.75 p-nitrophenyl-palmitate + H2O wild type lipase activity is rather low Paucimonas lemoignei p-nitrophenol + palmitate
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous PHB Paucimonas lemoignei poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O specific for amorphous poly(3-hydroxybutyrate) Paucimonas lemoignei poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PHA depolymerase
-
 Paucimonas lemoignei
3.1.1.75 PhaZ7
-
 Paucimonas lemoignei