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Literature summary extracted from
- Identification of the intracellular polyhydroxyalkanoate depolymerase gene of Paracoccus denitrificans and some properties of the gene product (2001), FEMS Microbiol. Lett., 196, 159-164.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.76 | gene phaZ, functional expression in Escherichia coli strain JM109 | Paracoccus denitrificans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.76 | bis(1-methylethyl) fluorophosphate | - |
Paracoccus denitrificans |  |
| 3.1.1.76 | Mg2+ | - |
Paracoccus denitrificans | |
| 3.1.1.76 | Triton X-100 | - |
Paracoccus denitrificans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 49300 | - |
x * 49300, recombinant enzyme, SDS-PAGE | Paracoccus denitrificans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.76 | poly(3-hydroxybutyrate) + H2O | Paracoccus denitrificans | degradation in vivo under carbon source starvation | oligo(3-hydroxybutyrate) | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.76 | Paracoccus denitrificans | - |
gene phaZ, strain ATCC 17741 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.76 | poly(3-hydroxybutyrate) + H2O | degradation in vivo under carbon source starvation | Paracoccus denitrificans | oligo(3-hydroxybutyrate) | - |
? | |
| 3.1.1.76 | poly(3-hydroxybutyrate) + H2O | - |
Paracoccus denitrificans | oligo(3-hydroxybutyrate) + D-(-)-3-hydroxybutyrate dimer | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.76 | ? | x * 49300, recombinant enzyme, SDS-PAGE | Paracoccus denitrificans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.76 | poly(3-hydroxyalkanoate) depolymerase | - |
Paracoccus denitrificans |
| 3.1.1.76 | polyhydroxyalkanoate depolymerase | - |
Paracoccus denitrificans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 30 | - |
assay at, recombinant enzyme | Paracoccus denitrificans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 9 | - |
assay at, recombinant enzyme | Paracoccus denitrificans |
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Release 2023.1 (January 2023)
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