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Literature summary extracted from

  • El-Kouhen, K.; Blangy, S.; Ortiz, E.; Gardies, A.M.; Ferte, N.; Arondel, V.
    Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases (2005), FEBS Lett., 579, 6067-6073.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene lip1, amino acid sequence comparison, expression of His-tagged enzyme in Escherichia coli, expression in insect cells using the baculovirus system Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 additional information construction of knockout mutant plants which do not show an altered phenotype during germination Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 additional information Arabidopsis thaliana the enzyme is involved in but not essential for breakdoen of stored triacylglycerides during germination ?
-
 ?
3.1.1.3 triolein + H2O Arabidopsis thaliana
-
 diolein + oleate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Arabidopsis thaliana Q71DJ5 precursor; gene lip1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein the enzyme contains 2 putative N-glycosylation sites Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 additional information tissue expression analysis, no expression in leaf, flower, root, and silique Arabidopsis thaliana
-
3.1.1.3 seed germinating, highest activity at the first germination day Arabidopsis thaliana
-
3.1.1.3 seedling
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 45
-
 recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,3-diolein + H2O low activity Arabidopsis thaliana 1-oleoyl-sn-glycerol + oleate
-
 ?
3.1.1.3 additional information the enzyme is involved in but not essential for breakdoen of stored triacylglycerides during germination Arabidopsis thaliana ?
-
 ?
3.1.1.3 additional information no activity with olein, digalactosyldiacylglycerol, galactosyldiacylglycerol, phosphatidylcholine, and cholesterol oleate Arabidopsis thaliana ?
-
 ?
3.1.1.3 tributyrin + H2O preferred substrate Arabidopsis thaliana dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprylin + H2O good substrate Arabidopsis thaliana dicaprylin + caprylate
-
 ?
3.1.1.3 triolein + H2O
-
 Arabidopsis thaliana diolein + oleate
-
 ?
3.1.1.3 triolein + H2O low activity Arabidopsis thaliana diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 More structure modelling Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 Lip1
-
 Arabidopsis thaliana
3.1.1.3 TAG lipase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 6
-
 recombinant enzyme Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 4 7.5 recombinant enzyme Arabidopsis thaliana