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Literature summary extracted from

  • Sender, P.D.; Martin, M.G.; Peiru, S.; Magni, C.
    Characterization of an oxaloacetate decarboxylase that belongs to the malic enzyme family (2004), FEBS Lett., 570, 217-222.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.112 expression in Escherichia coli M15 Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.112 2-oxoglutarate 2 mM inhibitor in presence of 10 mM Mn2+, 11% inhibition Lactococcus lactis
4.1.1.112 Ca2+ 2 mM inhibitor in presence of 1 mM Mn2+, 30% inhibition Lactococcus lactis
4.1.1.112 citrate 0.5 mM inhibitor or 2 mM inhibitor in presence of 10 mM Mn2+, 24% inhibition Lactococcus lactis
4.1.1.112 Cu2+ 0.5 mM inhibitor in presence of 1 mM Mn2+, complete inhibition Lactococcus lactis
4.1.1.112 EDTA 10 mM, complete inhibition Lactococcus lactis
4.1.1.112 fumarate 2 mM inhibitor in presence of 10 mM Mn2+, 21% inhibition Lactococcus lactis
4.1.1.112 malate 2 mM inhibitor in presence of 10 mM Mn2+, 54% inhibition Lactococcus lactis
4.1.1.112 malonate 0.5 mM inhibitor in presence of 10 mM Mn2+, 84% inhibition Lactococcus lactis
4.1.1.112 NAD+ 2 mM inhibitor in presence of 10 mM Mn2+, complete inhibition Lactococcus lactis
4.1.1.112 NADH 2 mM inhibitor in presence of 10 mM Mn2+, complete inhibition Lactococcus lactis
4.1.1.112 Ni2+ 2 mM inhibitor in presence of 1 mM Mn2+, 40% inhibition Lactococcus lactis
4.1.1.112 oxalate 0.5 mM inhibitor in presence of 10 mM Mn2+, 80% inhibition Lactococcus lactis
4.1.1.112 pyruvate 2 mM inhibitor in presence of 10 mM Mn2+, 8% inhibition Lactococcus lactis
4.1.1.112 succinate 2 mM inhibitor in presence of 10 mM Mn2+, 23% inhibition Lactococcus lactis
4.1.1.112 Zn2+ 0.5 mM inhibitor in presence of 1 mM Mn2+, complete inhibition Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.112 0.52
-
 oxaloacetate
-
 Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.112 Mg2+ activates Lactococcus lactis
4.1.1.112 Mn2+ dependent on, maximal activity in presence of 10 mM Mn2+ Lactococcus lactis
4.1.1.112 Ni2+ activates Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.112 Lactococcus lactis
-
 CRL264
-
4.1.1.112 Lactococcus lactis CRL264
-
 CRL264
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.112 recombinant Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.112 Oxaloacetate
-
 Lactococcus lactis Pyruvate + CO2
-
 ir
4.1.1.112 Oxaloacetate
-
 Lactococcus lactis CRL264 Pyruvate + CO2
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.112 50
-
-
 Lactococcus lactis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.112 58
-
 no activity above Lactococcus lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.112 3.5
-
 oxaloacetate
-
 Lactococcus lactis
4.1.1.112 20.26
-
 oxaloacetate
-
 Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.112 4.4
-
-
 Lactococcus lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.112 3.5 5.5 pH 3.5: about 55% of maximal activity, pH 5.5: about 40% of maximal activity Lactococcus lactis