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Literature summary extracted from

  • Araki, R.; Ali, M.K.; Sakka, M.; Kimura, T.; Sakka, K.; Ohmiya, K.
    Essential role of the family-22 carbohydrate-binding modules for beta-1,3-1,4-glucanase activity of Clostridium stercorarium Xyn10B (2004), FEBS Lett., 561, 155-158.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression of recombinant truncated proteins in Escherichia coli Thermoclostridium stercorarium

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.6 additional information construction of truncated proteins: a recombinant catalytic module polypeptide (rCD), a carbohydrate-binding module polypeptide composed of two family-22 carbohydrate-binding modules (rCBM) and a polypeptide composed of the family-22 carbohydrate-binding modules and the catalytic module (rCBM-CD). rCBM-CD is highly active towards beta-1,3-1,4-glucan, rCD is negligibly active towards the same substrate. The Vmax/KM value of rCBM-CD for beta-1,3-1,4-glucan is 7.8 times larger than that for oat-spelt xylan Thermoclostridium stercorarium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.6 additional information
-
 additional information
-
 Thermoclostridium stercorarium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Thermoclostridium stercorarium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6
-
 Thermoclostridium stercorarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 barley beta-glucan + H2O
-
 Thermoclostridium stercorarium ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Thermoclostridium stercorarium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 CBM-CD
-
 Thermoclostridium stercorarium
3.2.1.6 Xyn10B modular enzyme comprising two family-22 carbohydrate-binding modules (CBMs), a family-10 catalytic module of glycoside hydrolases, a family-9 carbohydrate-binding module, and two S-layer homologous modules consecutively from the N-terminus Thermoclostridium stercorarium