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Literature summary extracted from

  • Kochiwa, H.; Itaya, M.; Tomita, M.; Kanai, A.
    Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements (2006), FEBS J., 273, 420-429.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.4 genetic organization of the RNase H1-related gene family, different isozymes by alternative splicing, sequence comparisons of isozymes, overexpression of His-tagged isozymes in Escherichia coli strain BL21(DE3) Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.4 additional information
-
 additional information kinetics of isozymes Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.4 Mg2+ a divalent metal ion is required, dependent on the isozyme Caenorhabditis elegans
3.1.26.4 Mn2+ a divalent metal ion is required, dependent on the isozyme Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Caenorhabditis elegans
-
 different splicing variants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.4 recombinant His-tagged isozymes to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Caenorhabditis elegans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.4 additional information developmental stage-specific expression from L3 to adult stage of RNAse H1 isozymes with different substrate specificities and divalent cation requirements, overview Caenorhabditis elegans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.26.4 0.057
-
 isozyme RNase H1beta, with Mn2+ Caenorhabditis elegans
3.1.26.4 0.07
-
 isozyme RNase H1beta, with Mg2+ Caenorhabditis elegans
3.1.26.4 2.1
-
 isozyme RNase H1alpha, with Mn2+ Caenorhabditis elegans
3.1.26.4 7
-
 isozyme RNase H1alpha, with Mg2+ Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 M13 DNA-RNA hybrid + H2O the enzyme degrades the RNA moiety of the heteroduplex Caenorhabditis elegans ?
-
 ?
3.1.26.4 additional information stage-specific expression of RNAse H1 isozymes with different substrate specificities and divalent cation requirements, claevage specificties, overview Caenorhabditis elegans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 ribonuclease H1
-
 Caenorhabditis elegans
3.1.26.4 RNase H1
-
 Caenorhabditis elegans