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Literature summary extracted from

  • Mayer, C.; Vocadlo, D.J.; Mah, M.; Rupitz, K.; Stoll, D.; Warren, R.A.; Withers, S.G.
    Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi (2006), FEBS J., 273, 2929-2941.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 N-acetyl-glucosamine 0.05% (w/v) Cellulomonas fimi
3.2.1.52 N-acetylglucosamine 0.05% (w/v) Cellulomonas fimi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Escherichia coli BL21(DE3) cells Cellulomonas fimi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.0027
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi
3.2.1.52 0.053
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 4-nitrophenyl-beta-N-acetyl-D-glucosaminide Cellulomonas fimi
3.2.1.52 0.066
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 4-nitrophenyl-beta-N-acetyl-D-galactosaminide Cellulomonas fimi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 54190
-
 electrospray mass spectroscopy Cellulomonas fimi
3.2.1.52 60970
-
 electrospray mass spectroscopy Cellulomonas fimi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Cellulomonas fimi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 His-bind resin column chromatography Cellulomonas fimi

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, glycerol (20% final concentration) and phosphate buffer at pH 7.3, several months, loss of catalytic activity in diluted buffers within a day at 4 °C Cellulomonas fimi
3.2.1.52 4°C, elution buffer (20 mM sodium phosphate, 80 mM imidazole pH 7.5 and 300 mM NaCl), several months Cellulomonas fimi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 (25R)-3beta-hydroxycholest-5-en-27-oate + H2O
-
 Cellulomonas fimi ?
-
 ?
3.2.1.52 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Cellulomonas fimi 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Cellulomonas fimi 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Cellulomonas fimi 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 3-beta-N-acetylglucosaminidase
-
 Cellulomonas fimi
3.2.1.52 beta-glucosidase
-
 Cellulomonas fimi
3.2.1.52 beta-N-acetylglucosaminidase
-
 Cellulomonas fimi
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Cellulomonas fimi
3.2.1.52 Hex20
-
 Cellulomonas fimi
3.2.1.52 Nag3
-
 Cellulomonas fimi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.52 0.18
-
 4-nitrophenyl beta-N-acetyl-D-glucosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi
3.2.1.52 129
-
 4-nitrophenyl beta-N-acetyl-D-galactosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi
3.2.1.52 482
-
 4-nitrophenyl-beta-N-acetyl-D-glucosaminide 50 mM sodium phosphate buffer (pH 7.08) at 25 °C Cellulomonas fimi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 7.3
-
-
 Cellulomonas fimi
3.2.1.52 8
-
-
 Cellulomonas fimi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 6 9.5 inactivation above pH 9.5 Cellulomonas fimi
3.2.1.52 6.8 8.4
-
 Cellulomonas fimi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 6 9.5 inactivation above pH 9.5 Cellulomonas fimi
3.2.1.52 6.8 8.4
-
 Cellulomonas fimi