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Literature summary extracted from

  • Yeung, D.T.; Lenz, D.E.; Cerasoli, D.M.
    Analysis of active-site amino-acid residues of human serum paraoxonase using competitive substrates (2005), FEBS J., 272, 2225-2230.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.2 F222Y hydrolysis of phenyl acetate, but not of paraoxon Homo sapiens
3.1.1.2 H115W no hydrolysis of phenyl acetate, but hydrolysis of paraoxon. Phenyl acetate can act as an inhibitor Homo sapiens
3.1.1.2 H115W/N133S no hydrolysis of phenyl acetate, but hydrolysis of paraoxon Homo sapiens
3.1.8.1 F222Y hydrolysis of phenyl acetate, but not of paraoxon Homo sapiens
3.1.8.1 H115W no hydrolysis of phenyl acetate, but hydrolysis of paraoxon. Phenyl acetate can act as an inhibitor Homo sapiens
3.1.8.1 H115W/N133S no hydrolysis of phenyl acetate, but hydrolysis of paraoxon Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 diisopropylfluorophosphate
-
 Homo sapiens
3.1.8.1 diisopropylfluorophosphate
-
 Homo sapiens
3.1.8.1 phenyl acetate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.61
-
 phenyl acetate pH 8.0, 25°C, wild-type enzyme Homo sapiens
3.1.1.2 0.81
-
 phenyl acetate pH 8.0, 25°C, mutant enzyme F222Y Homo sapiens
3.1.8.1 0.19
-
 paraoxon pH 10.5, 25°C, mutant enzyme H115W/N133S Homo sapiens
3.1.8.1 0.25
-
 paraoxon pH 10.5, 25°C, wild-type enzyme Homo sapiens
3.1.8.1 0.31
-
 paraoxon pH 8.5, 25°C, wild-type enzyme Homo sapiens
3.1.8.1 0.43
-
 paraoxon pH 10.5, 25°C, mutant enzyme H115W Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens P27169
-
-
3.1.8.1 Homo sapiens P27169
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.1.2 phenylacetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.8.1 paraoxon + H2O
-
 Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 PON1
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.2 0.52
-
 diisopropylfluorophosphate pH 8.0, 25°C, wild-type enzyme, substrate phenyl acetate Homo sapiens
3.1.8.1 0.44
-
 diisopropylfluorophosphate pH 8.5, 25°C, wild-type enzyme, substrate paraoxon Homo sapiens
3.1.8.1 0.47
-
 phenyl acetate pH 8.5, 25°C, wild-type enzyme, substrate paraoxon Homo sapiens
3.1.8.1 0.91
-
 phenyl acetate pH 10.5, 25°C, wild-type enzyme, substrate paraoxon Homo sapiens
3.1.8.1 1.95
-
 phenyl acetate pH 10.5, 25°C, mutant enzyme H115W, substrate paraoxon Homo sapiens