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Literature summary extracted from

  • Akeboshi, H.; Tonozuka, T.
    Insights into the reaction mechanism of glycosol hydrolase family 49. Site-directed mutagenesis and substrate preference of isopullulanase (2004), Eur. J. Biochem., 271, 4420-4427.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.57 D353N enzyme shows no activity with pullulan and panose as substrate Aspergillus niger
3.2.1.57 D372N enzyme shows no activity with pullulan and panose as substrate Aspergillus niger
3.2.1.57 D373N enzyme shows no activity with pullulan and panose as substrate Aspergillus niger
3.2.1.57 E273N 45% of wild-type activity with pullulan as substrate, 74% of wild-type activity with panose as substrate Aspergillus niger
3.2.1.57 E356Q 38% of wild-type activity with pullulan as substrate, 50% of wild-type activity with panose as substrate Aspergillus niger
3.2.1.57 W240F 130% of wild-type activity with pullulan as substrate, 160% of wild-type activity with panose as substrate Aspergillus niger
3.2.1.57 W31F 38% of wild-type activity with pullulan as substrate, 140% of wild-type activity with panose as substrate Aspergillus niger
3.2.1.57 W32F 90% of wild-type activity with pullulan as substrate, 120% of wild-type activity with panose as substrate Aspergillus niger
3.2.1.57 W402F 0.4% of wild-type activity with pullulan as substrate, 0.1% of wild-type activity with panose as substrate Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.57 160
-
 panose pH 3.5, 40°C, wild-type enzyme Aspergillus niger
3.2.1.57 920
-
 panose pH 3.5, 40°C, mutant enzyme W402F Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.57 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.57 wild-type and mutant enzymes Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.57 panose + H2O
-
 Aspergillus niger isomaltose + glucose
-
 ?
3.2.1.57 pullulan + H2O
-
 Aspergillus niger isopanose + ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.57 0.36
-
 panose mutant enzyme W402F Aspergillus niger
3.2.1.57 180
-
 panose wild-type enzyme Aspergillus niger