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Literature summary extracted from

  • Pontoppidan, B.; Ekbom, B.; Eriksson, S.; Meijer, J.
    Purification and characterization of myrosinase from the cabbage aphid (Brevicoryne brassicae), a brassica herbivore (2001), Eur. J. Biochem., 268, 1041-1048.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.147 some loss of activity upon freezing Brevicoryne brassicae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.147 ascorbate 0.3 mM, strong activation Brevicoryne brassicae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.41
-
 sinigrin 37°C, pH 4.5 Brevicoryne brassicae
3.2.1.147 0.52
-
 glucotropaeolin 37°C, pH 4.5 Brevicoryne brassicae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.147 126000
-
 sucrose density gradient sedimentation Brevicoryne brassicae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Brevicoryne brassicae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147
-
 Brevicoryne brassicae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.147 28
-
-
 Brevicoryne brassicae

Storage Stability

EC Number Storage Stability Organism
3.2.1.147 4°C, stable for more than 6 months Brevicoryne brassicae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 glucotropaeolin + H2O
-
 Brevicoryne brassicae ?
-
 ?
3.2.1.147 p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Brevicoryne brassicae p-nitrophenol + D-glucose
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brevicoryne brassicae D-glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.147 dimer 2 * 57000-58000, SDS-PAGE Brevicoryne brassicae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.147 40
-
 hydrolysis of sinigrin Brevicoryne brassicae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.147 3 6 sinigrin 37°C, pH 4.5 Brevicoryne brassicae
3.2.1.147 22.8
-
 glucotropaeolin 37°C, pH 4.5 Brevicoryne brassicae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.147 Brevicoryne brassicae isoelectric focusing, pH-range 3-9
-
 4.9