Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gusakov, A.V.; Sinitsyn, A.P.; Salanovich, T.N.; Bukhtojarov, F.E.; Markov, A.V.; Ustinov, B.B.; van Zeijl, C.; Punt, P.; Burlingame, R.
    Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense (2005), Enzyme Microb. Technol., 36, 57-69.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 gene cbh1, DNA and amino acid sequence determination Chrysosporium lucknowense

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 additional information isolation of the truncated catalytic core enzyme form 2 lacking the cellulose binding module and the major part of glycosylated linker from a mutant strain Chrysosporium lucknowense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.176 52000
-
 x * 65000, full-length enzyme form 1, SDS-PAGE, x * 52000, truncated catalytic core enzyme form 2, SDS-PAGE Chrysosporium lucknowense
3.2.1.176 65000
-
 x * 65000, full-length enzyme form 1, SDS-PAGE, x * 52000, truncated catalytic core enzyme form 2, SDS-PAGE Chrysosporium lucknowense

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Chrysosporium lucknowense
-
 gene cbh1, wild-type and mutant industrial strain, full-length and mutant enzyme forms
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 avicel + H2O
-
 Chrysosporium lucknowense ?
-
 ?
3.2.1.176 cellulose + H2O from cotton Chrysosporium lucknowense beta-D-cellobiose
-
 ?
3.2.1.176 additional information the full-length and mutant enzyme forms show similar substrate specificities Chrysosporium lucknowense ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.176 ? x * 65000, full-length enzyme form 1, SDS-PAGE, x * 52000, truncated catalytic core enzyme form 2, SDS-PAGE Chrysosporium lucknowense
3.2.1.176 More peptide sequencing of Cel7A and CBH I Chrysosporium lucknowense

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 CBH I
-
 Chrysosporium lucknowense
3.2.1.176 Cel7A
-
 Chrysosporium lucknowense
3.2.1.176 cellobiohydrolase I
-
 Chrysosporium lucknowense
3.2.1.176 More the enzyme Cel7A probably belongs to the cellobiohydrolase family 7 Chrysosporium lucknowense

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 additional information
-
 the full-length enzyme form is more thermostable than the truncated mutant enzyme form Chrysosporium lucknowense
3.2.1.176 50
-
 24 h, stable, full-length and mutant enzyme forms Chrysosporium lucknowense
3.2.1.176 60
-
 7 h, 90% remaining activity of the full-length enzyme form Chrysosporium lucknowense
3.2.1.176 65
-
 3 h, 50% remaining activity of the full-length enzyme form Chrysosporium lucknowense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 5 5.5 full-length and mutant enzyme forms Chrysosporium lucknowense

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.176 Chrysosporium lucknowense full-length and mutant enzyme forms
-
 4.5