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Literature summary extracted from

  • Birgisson, H.; Hreggvidsson, G.O.; Fridjonsson, O.H.; Mort, A.; Kristjansson, J.K.; Mattiasson, B.
    Two new thermostable alpha-L-rhamnosidases from a novel thermophilic bacterium (2004), Enzyme Microb. Technol., 34, 561-571.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.40 nutrition alpha-L-rhamnosidases are used in wine production Thermomicrobia bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 gene rhmA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Thermomicrobia bacterium
3.2.1.40 gene rhmB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Thermomicrobia bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 D-glucose about 500 mM D-glucose concentration in grape juice inhibits RhmA by 39%; about 500 mM D-glucose concentration in grape juice inhibits RhmB by 38% Thermomicrobia bacterium
3.2.1.40 ethanol at 12% v/v, 51% inhibition of RhmB; at 12% v/v, 60% inhibition of RhmA Thermomicrobia bacterium
3.2.1.40 L-rhamnose complete inhibition, competitive product inhibition; complete inhibition, competitive product inhibition Thermomicrobia bacterium
3.2.1.40 Zn2+ 30-35% inhibition at 2 mM, reversible by 10 mM EDTA Thermomicrobia bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 additional information
-
 additional information kinetics Thermomicrobia bacterium
3.2.1.40 0.46
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 7.5, 50°C, recombinant RhmA Thermomicrobia bacterium
3.2.1.40 0.66
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 7.0, 50°C, recombinant RhmB Thermomicrobia bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.40 additional information no effect by EDTA, Mg2+, Ca2+, and Mn2+ on RhmB Thermomicrobia bacterium
3.2.1.40 additional information no effect by EDTA, Zn2+, Mg2+, Ca2+, and Mn2+ on RhmA Thermomicrobia bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 104000
-
 2 * 104000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium
3.2.1.40 107000
-
 2 * 107000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium
3.2.1.40 210000
-
 recombinant enzyme, gel filtration Thermomicrobia bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Thermomicrobia bacterium Q6RCI8 gene rhmB, isozyme RhmB
-
3.2.1.40 Thermomicrobia bacterium Q6RCI9 gene rhmA, isozyme RhmA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 recombinant enzyme 63fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography Thermomicrobia bacterium
3.2.1.40 recombinant enzyme 85fold from Escherichia coli by heat treatment at 60°C, hydrophobic interaction and ion exchange chromatography Thermomicrobia bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 108.9
-
 purified recombinant enzyme Thermomicrobia bacterium
3.2.1.40 263.9
-
 purified recombinant enzyme Thermomicrobia bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Thermomicrobia bacterium 4-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 hesperidin + H2O cleavage of the alpha-1,6-linkage Thermomicrobia bacterium ? + alpha-L-rhamnose
-
 ?
3.2.1.40 additional information no activity with quercitrin Thermomicrobia bacterium ?
-
 ?
3.2.1.40 additional information no activity with rutin and quercitrin Thermomicrobia bacterium ?
-
 ?
3.2.1.40 naringin + H2O preferred substrate, cleavage of the alpha-1,2-linkage Thermomicrobia bacterium ? + alpha-L-rhamnose
-
 ?
3.2.1.40 rhamnogalacturonan tetramer + H2O
-
 Thermomicrobia bacterium ?
-
 ?
3.2.1.40 rutin + H2O low activity, cleavage of the alpha-1,6-linkage Thermomicrobia bacterium ? + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 dimer 2 * 104000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium
3.2.1.40 dimer 2 * 107000, recombinant enzyme, SDS-PAGE Thermomicrobia bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 alpha-L-rhamnosidase A
-
 Thermomicrobia bacterium
3.2.1.40 alpha-L-rhamnosidase B
-
 Thermomicrobia bacterium
3.2.1.40 RhmA
-
 Thermomicrobia bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 70
-
-
 Thermomicrobia bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 40 80 low activity above Thermomicrobia bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.40 40
-
 completely stable for 24 h at pH 5.0-6.9 Thermomicrobia bacterium
3.2.1.40 40
-
 loss of 22% activity within 24 h at pH 7.9 Thermomicrobia bacterium
3.2.1.40 60
-
 20-30% remaining activity after 24 h at pH 5.0-6.9 Thermomicrobia bacterium
3.2.1.40 60
-
 20-30% remaining activity after 24 h at pH 7.9 Thermomicrobia bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 5 6.9
-
 Thermomicrobia bacterium
3.2.1.40 7.9
-
-
 Thermomicrobia bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 4 7.9 50% of maximal activity at pH 4.0 and pH 7.9 Thermomicrobia bacterium
3.2.1.40 5 8.7 50% of maximal activity at pH 5.0 and pH 8.7 Thermomicrobia bacterium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.40 1.28
-
 L-rhamnose pH 7.5, 50°C, recombinant RhmA, versus 4-nitrophenyl alpha-L-rhamnopyranoside Thermomicrobia bacterium
3.2.1.40 4.65
-
 L-rhamnose pH 7.0, 50°C, recombinant RhmB, versus 4-nitrophenyl alpha-L-rhamnopyranoside Thermomicrobia bacterium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.40 Thermomicrobia bacterium sequence calculation
-
 4.5
3.2.1.40 Thermomicrobia bacterium sequence calculation
-
 4.6