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Literature summary extracted from

  • Nieder, V.; Kutzer, M.; Kren, V.; Gallego, R.G.; Kamerling, J.P.; Elling, L.
    Screening and characterization of beta-N-acetylhexosaminidases for the synthesis of nucleotide-activated disaccharides (2004), Enzyme Microb. Technol., 34, 407-414.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N-acetyl-D-galactosamine
-
 Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 3.21
-
 4-nitrophenyl beta-N-acetyl-D-galactosaminide
-
 Trichoderma harzianum
3.2.1.52 3.44
-
 4-nitrophenyl beta-N-acetyl-D-galactosaminide in presence of 200 mM heptakis-(2,6-di-O-methyl)-beta-cyclodextrin Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Aspergillus flavipes
-
-
-
3.2.1.52 Aspergillus flavus
-
-
-
3.2.1.52 Aspergillus oryzae
-
-
-
3.2.1.52 Aspergillus parasiticus
-
-
-
3.2.1.52 Aspergillus tamarii
-
-
-
3.2.1.52 Penicillium oxalicum
-
-
-
3.2.1.52 Trichoderma harzianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 ammonium sulfate fractionation Aspergillus flavus
3.2.1.52 ammonium sulfate fractionation Aspergillus oryzae
3.2.1.52 ammonium sulfate fractionation Aspergillus parasiticus
3.2.1.52 ammonium sulfate fractionation Trichoderma harzianum
3.2.1.52 ammonium sulfate fractionation Penicillium oxalicum
3.2.1.52 ammonium sulfate fractionation Aspergillus flavipes
3.2.1.52 ammonium sulfate fractionation Aspergillus tamarii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 21
-
-
 Aspergillus parasiticus
3.2.1.52 30
-
-
 Penicillium oxalicum
3.2.1.52 30
-
-
 Aspergillus tamarii
3.2.1.52 34
-
-
 Aspergillus flavus
3.2.1.52 44
-
-
 Trichoderma harzianum
3.2.1.52 49
-
-
 Aspergillus oryzae
3.2.1.52 55
-
-
 Aspergillus flavipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-galactosaminide + UDP-N-acetyl-D-glucosamine + H2O
-
 Trichoderma harzianum UDP-(4-O-N-acetyl-beta-D-galactosaminyl)-N-acetyl-alpha-D-glucosamine + 4-nitrophenol
-
 ir
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Trichoderma harzianum 4-nitrophenol + N-acetyl-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Aspergillus flavus 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Aspergillus oryzae 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Aspergillus parasiticus 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Aspergillus flavipes 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
-
 Aspergillus tamarii 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
-
 Trichoderma harzianum 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 p-nitrophenyl-beta-N-acetyl-D-glucosaminide + H2O
-
 Penicillium oxalicum N-acetyl-D-glucosamine + p-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Aspergillus flavus
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Aspergillus oryzae
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Aspergillus parasiticus
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Trichoderma harzianum
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Penicillium oxalicum
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Aspergillus flavipes
3.2.1.52 Beta-N-acetylhexosaminidase
-
 Aspergillus tamarii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 30
-
-
 Aspergillus flavus
3.2.1.52 30
-
-
 Aspergillus oryzae
3.2.1.52 30
-
-
 Aspergillus parasiticus
3.2.1.52 30
-
-
 Trichoderma harzianum
3.2.1.52 30
-
-
 Penicillium oxalicum
3.2.1.52 30
-
-
 Aspergillus flavipes
3.2.1.52 30
-
-
 Aspergillus tamarii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 6.5
-
-
 Aspergillus flavus
3.2.1.52 6.5
-
-
 Aspergillus oryzae
3.2.1.52 6.5
-
-
 Aspergillus parasiticus
3.2.1.52 6.5
-
-
 Trichoderma harzianum
3.2.1.52 6.5
-
-
 Penicillium oxalicum
3.2.1.52 6.5
-
-
 Aspergillus flavipes
3.2.1.52 6.5
-
-
 Aspergillus tamarii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.52 0.21
-
 N-acetyl-D-galactosamine in presence of 200 mM heptakis-(2,6-di-O-methyl)-beta-cyclodextrin Trichoderma harzianum
3.2.1.52 1.19
-
 N-acetyl-D-galactosamine
-
 Trichoderma harzianum