EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.75 | expression in Escherichia coli | Ralstonia pickettii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.22 | 0.35 | - |
(R)-3-((R)-3-hydroxybutanoloxy)butanoate | - |
Cupriavidus necator | |
3.1.1.22 | 0.61 | - |
(R)-3-((R)-3-hydroxybutanoloxy)butanoate | - |
Ralstonia pickettii | |
3.1.1.22 | 0.7 | - |
(R)-3-((R)-3-hydroxybutanoloxy)butanoate | - |
Acidovorax sp. | |
3.1.1.75 | 0.34 | - |
cyclic [(R)-3-hydroxybutanoate]5 | cyclic 3-hydroxybutyrate oligomer | Acidovorax sp. | |
3.1.1.75 | 0.36 | - |
[(R)-3-hydroxybutanoate]6 | cyclic 3-hydroxybutyrate oligomer | Acidovorax sp. | |
3.1.1.75 | 0.56 | - |
[(R)-3-hydroxybutanoate]5 | linear 3-hydroxybutyrateoligomer | Acidovorax sp. | |
3.1.1.75 | 0.67 | - |
[(R)-3-hydroxybutanoate]4 | linear 3-hydroxybutyrate oligomer | Acidovorax sp. | |
3.1.1.75 | 0.84 | - |
cyclic [(R)-3-hydroxybutanoate]3 | cyclic 3-hydroxybutyrate oligomer | Acidovorax sp. | |
3.1.1.75 | 1.3 | - |
[(R)-3-hydroxybutanoate]5 | linear 3-hydroxybutyrate oligomer | Cupriavidus necator | |
3.1.1.75 | 3.1 | - |
[(R)-3-hydroxybutanoate]4 | linear 3-hydroxybutyrate oligomer | Cupriavidus necator | |
3.1.1.75 | 4.1 | - |
[(R)-3-hydroxybutanoate]3 | linear 3-hydroxybutyrate oligomer | Acidovorax sp. | |
3.1.1.75 | 4.6 | - |
cyclic [(R)-3-hydroxybutanoate]5 | cyclic 3-hydroxybutyrate oligomer | Cupriavidus necator | |
3.1.1.75 | 9.7 | - |
[(R)-3-hydroxybutanoate]3 | linear 3-hydroxybutyrate oligomer | Cupriavidus necator |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.22 | extracellular | - |
Ralstonia pickettii | - |
- |
3.1.1.22 | intracellular | - |
Cupriavidus necator | 5622 | - |
3.1.1.22 | intracellular | - |
Acidovorax sp. | 5622 | - |
3.1.1.75 | extracellular | - |
Ralstonia pickettii | - |
- |
3.1.1.75 | extracellular | - |
Acidovorax sp. | - |
- |
3.1.1.75 | intracellular | - |
Cupriavidus necator | 5622 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | Cupriavidus necator | involved in the degradation of poly-3-hydroxybutyrate (PHB) | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | Ralstonia pickettii | involved in the degradation of poly-3-hydroxybutyrate (PHB) | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | Acidovorax sp. | involved in the degradation of poly-3-hydroxybutyrate (PHB) | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | involved in the degradation of poly-3-hydroxybutyrate (PHB) | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | Ralstonia pickettii T1 | involved in the degradation of poly-3-hydroxybutyrate (PHB) | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | additional information | Cupriavidus necator | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | ? | - |
? | |
3.1.1.22 | additional information | Ralstonia pickettii | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | ? | - |
? | |
3.1.1.22 | additional information | Acidovorax sp. | hydrolyzes exclusively 3-hydroxybutyrate-dimers and trimers, no hydrolysis of longer linear or cyclic oligomers | ? | - |
? | |
3.1.1.22 | additional information | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | ? | - |
? | |
3.1.1.22 | additional information | Ralstonia pickettii T1 | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | ? | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | Cupriavidus necator | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | Ralstonia pickettii | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | Acidovorax sp. | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | Ralstonia pickettii T1 | - |
poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.22 | Acidovorax sp. | - |
- |
- |
3.1.1.22 | Cupriavidus necator | - |
- |
- |
3.1.1.22 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
3.1.1.22 | Ralstonia pickettii | - |
- |
- |
3.1.1.22 | Ralstonia pickettii T1 | - |
- |
- |
3.1.1.75 | Acidovorax sp. | - |
- |
- |
3.1.1.75 | Cupriavidus necator | - |
- |
- |
3.1.1.75 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
3.1.1.75 | Ralstonia pickettii | - |
- |
- |
3.1.1.75 | Ralstonia pickettii T1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.75 | - |
Ralstonia pickettii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.75 | culture fluid | - |
Ralstonia pickettii | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | - |
Cupriavidus necator | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | - |
Ralstonia pickettii | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | - |
Acidovorax sp. | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | involved in the degradation of poly-3-hydroxybutyrate (PHB) | Cupriavidus necator | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | involved in the degradation of poly-3-hydroxybutyrate (PHB) | Ralstonia pickettii | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | involved in the degradation of poly-3-hydroxybutyrate (PHB) | Acidovorax sp. | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | involved in the degradation of poly-3-hydroxybutyrate (PHB) | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | - |
Ralstonia pickettii T1 | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | (R)-3-((R)-3-hydroxybutanoloxy)butanoate + H2O | involved in the degradation of poly-3-hydroxybutyrate (PHB) | Ralstonia pickettii T1 | (R)-3-hydroxybutanoate | - |
? | |
3.1.1.22 | additional information | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | Cupriavidus necator | ? | - |
? | |
3.1.1.22 | additional information | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | Ralstonia pickettii | ? | - |
? | |
3.1.1.22 | additional information | hydrolyzes exclusively 3-hydroxybutyrate-dimers and trimers, no hydrolysis of longer linear or cyclic oligomers | Acidovorax sp. | ? | - |
? | |
3.1.1.22 | additional information | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
3.1.1.22 | additional information | hydrolyzes 3-hydroxybutyrate-dimers, trimers, tetramers, pentamers and cyclic trimers, pentamers and hexamers at a similar efficiency | Ralstonia pickettii T1 | ? | - |
? | |
3.1.1.75 | cyclic [(R)-3-hydroxybutanoate]3 + H2O | - |
Acidovorax sp. | ? | - |
? | |
3.1.1.75 | cyclic [(R)-3-hydroxybutanoate]5 + H2O | - |
Cupriavidus necator | ? | - |
? | |
3.1.1.75 | cyclic [(R)-3-hydroxybutanoate]5 + H2O | - |
Acidovorax sp. | ? | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | - |
Cupriavidus necator | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | - |
Ralstonia pickettii | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | - |
Acidovorax sp. | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | linear 3-hydroxbutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxbutyrate trimer, pentamer, hexamer | Ralstonia pickettii | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | linear 3-hydroxybutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxybutyrate trimer, pentamer, hexamer | Cupriavidus necator | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | linear 3-hydroxybutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxybutyrate trimer, pentamer, hexamer | Acidovorax sp. | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | linear 3-hydroxybutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxybutyrate trimer, pentamer, hexamer | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | - |
Ralstonia pickettii T1 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | linear 3-hydroxbutyrate dimer, trimer, tetramer, pentamer, cyclic 3-hydroxbutyrate trimer, pentamer, hexamer | Ralstonia pickettii T1 | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? | |
3.1.1.75 | [(R)-3-hydroxybutanoate]3 + H2O | - |
Cupriavidus necator | ? | - |
? | |
3.1.1.75 | [(R)-3-hydroxybutanoate]3 + H2O | - |
Acidovorax sp. | ? | - |
? | |
3.1.1.75 | [(R)-3-hydroxybutanoate]4 + H2O | - |
Cupriavidus necator | ? | - |
? | |
3.1.1.75 | [(R)-3-hydroxybutanoate]4 + H2O | - |
Acidovorax sp. | ? | - |
? | |
3.1.1.75 | [(R)-3-hydroxybutanoate]5 + H2O | - |
Cupriavidus necator | ? | - |
? | |
3.1.1.75 | [(R)-3-hydroxybutanoate]5 + H2O | - |
Acidovorax sp. | ? | - |
? | |
3.1.1.75 | [(R)-3-hydroxybutanoate]6 + H2O | - |
Acidovorax sp. | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.22 | 3-HB-oligomer hydrolase | - |
Cupriavidus necator |
3.1.1.22 | 3-HB-oligomer hydrolase | - |
Ralstonia pickettii |
3.1.1.22 | 3-HB-oligomer hydrolase | - |
Acidovorax sp. |
3.1.1.22 | 3HBOH | - |
Cupriavidus necator |
3.1.1.22 | 3HBOH | - |
Ralstonia pickettii |
3.1.1.22 | 3HBOH | - |
Acidovorax sp. |
3.1.1.75 | PHBDP | - |
Cupriavidus necator |
3.1.1.75 | PHBDP | - |
Ralstonia pickettii |
3.1.1.75 | PHBDP | - |
Acidovorax sp. |
3.1.1.75 | poly(3-hydroxybutyrate) depolymerase | - |
Cupriavidus necator |
3.1.1.75 | poly(3-hydroxybutyrate) depolymerase | - |
Ralstonia pickettii |
3.1.1.75 | poly(3-hydroxybutyrate) depolymerase | - |
Acidovorax sp. |