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Literature summary extracted from

  • Umetsu, H.; Arai, M.; Ota, T.; Kudo, R.; Sugiura, H.; Ishiyama, H.; Sasaki, K.
    Purification and properties of an aminopeptidase from the mid-gut gland of scallop (Patinopecten yessoensis) (2003), Comp. Biochem. Physiol. B, 136B, 935-942.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline the inhibition is completely reversible by Zn2+, but not by other metal ions such as Mg2+ or Ca2+, partially by Co2+ or Mn2+ Mizuhopecten yessoensis
3.4.11.2 actinonin slight inhibition Mizuhopecten yessoensis
3.4.11.2 arphamenine A slight inhibition Mizuhopecten yessoensis
3.4.11.2 bestatin
-
 Mizuhopecten yessoensis
3.4.11.2 EDTA
-
 Mizuhopecten yessoensis
3.4.11.2 additional information no inhibition by antipain, E64, leupeptin, chymostatin, PCMB, PMSF, amastatin, and puromycin Mizuhopecten yessoensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.011
-
 L-Ala-7-amido-4-methylcoumarin pH 7.0, 30°C Mizuhopecten yessoensis
3.4.11.2 0.032
-
 L-Ala-4-nitroanilide pH 7.0, 30°C Mizuhopecten yessoensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 59000
-
 gel filtration Mizuhopecten yessoensis
3.4.11.2 61000
-
 1 * 61000, SDS-PAGE Mizuhopecten yessoensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Mizuhopecten yessoensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 native enzyme 340fold from midgut gland by acetone extraction, ammonium sulfate fractionation, ion exchange chromatography, isoelectric focusing, and hydrophobic interaction chromatography Mizuhopecten yessoensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 midgut
-
 Mizuhopecten yessoensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.2 additional information
-
-
 Mizuhopecten yessoensis
3.4.11.2 53
-
 purified enzyme Mizuhopecten yessoensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Ala-Phe-Tyr-Glu + H2O sequential release of N-terminal amino acids Mizuhopecten yessoensis Ala + Phe + Tyr + Glu
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Mizuhopecten yessoensis L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-7-amido-4-methylcoumarin + H2O
-
 Mizuhopecten yessoensis L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Leu-4-nitroanilide + H2O
-
 Mizuhopecten yessoensis L-Leu + 4-nitroaniline
-
 ?
3.4.11.2 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Mizuhopecten yessoensis L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Met-4-nitroanilide + H2O
-
 Mizuhopecten yessoensis L-Met + 4-nitroaniline
-
 ?
3.4.11.2 L-Met-7-amido-4-methylcoumarin + H2O
-
 Mizuhopecten yessoensis L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 additional information substrate specificity, the enzyme prefers substrates with Ala or Met residues at the N-terminus, overview Mizuhopecten yessoensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 monomer 1 * 61000, SDS-PAGE Mizuhopecten yessoensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 30
-
 assay at Mizuhopecten yessoensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.2 0.04 1.97 L-Ala-7-amido-4-methylcoumarin pH 7.0, 30°C Mizuhopecten yessoensis
3.4.11.2 9.49
-
 L-Ala-7-amido-4-methylcoumarin pH 7.0, 30°C Mizuhopecten yessoensis
3.4.11.2 29.5
-
 L-Ala-4-nitroanilide pH 7.0, 30°C Mizuhopecten yessoensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7
-
-
 Mizuhopecten yessoensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.2 4 8 stable, purified enzyme Mizuhopecten yessoensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.2 Mizuhopecten yessoensis isoelectric focusing
-
 5.2