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Literature summary extracted from

  • Michelin, K.; Wajner, A.; Goulart, L.d.S.; Fachel, A.A.; Pereira, M.L.; de Mello, A.S.; Souza, F.T.; Pires, R.F.; Giugliani, R.; Coelho, J.C.
    Biochemical study on beta-glucosidase in individuals with Gaucher's disease and normal subjects (2004), Clin. Chim. Acta, 343, 145-153.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 additional information
-
 additional information
-
 Homo sapiens
3.2.1.21 3.64
-
 4-methylumbelliferyl beta-D-glucoside pH 5.4, 37°C, Gaucher's disease enzyme from leukocytes Homo sapiens
3.2.1.21 4.13
-
 4-methylumbelliferyl beta-D-glucoside pH 5.4, 37°C, healthy human enzyme from leukocytes Homo sapiens
3.2.1.21 6.18
-
 4-methylumbelliferyl beta-D-glucoside pH 5.4, 37°C, healthy human enzyme from fibroblasts Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 additional information Homo sapiens enzyme deficiency causes the Gaucher's disease, the enzyme participates in degradation of glycosphingolipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Homo sapiens
-
 healthy humans and Gaucher's disease patients
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 fibroblast from healthy humans and Gaucher's disease patients Homo sapiens
-
3.2.1.21 leukocyte from healthy humans and Gaucher's disease patients Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl beta-D-glucoside + H2O
-
 Homo sapiens 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 additional information enzyme deficiency causes the Gaucher's disease, the enzyme participates in degradation of glycosphingolipids Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 half-life of the healthy leukocyte enzyme is 3.5 min, of the healthy fibroblast 3 min, and half-life of the Gaucher's disease enzyme from leukocyte is about 6 min, and from fibroblast 3 min, thermostability, overview Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.9 5.3 healthy human enzyme Homo sapiens
3.2.1.21 5.2 5.4 Gaucher's disease enzyme Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 10.3
-
 inactivation Homo sapiens