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Literature summary extracted from

  • Wang, W.; Liu, G.; Yamashita, K.; Manabe, M.; Kodama, H.
    Characteristics of prolinase against various iminodipeptides in erythrocyte lysates from a normal human and a patient with prolidase deficiency (2004), Clin. Chem. Lab. Med., 42, 1102-1108.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.13.9 glycine activation of activity against substrates L-Pro-L-Ala, L-Pro-L-Val, L-Pro-L-Met, and L-Pro-L-Asp Homo sapiens
3.4.13.9 MnCl2 stimulation of activity against substrates L-Pro-Gly, L-Pro-L-Glu, L-Pro-L-Leu, L-Pro-L-Ser, and L-Pro-L-Phe, inhibitory to hydrolysis of substrates L-Pro-L-Ala, L-Pro-L-Val, L-Pro-L-Met, and L-Pro-L-Asp Homo sapiens
3.4.13.18 Ala prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated Homo sapiens
3.4.13.18 D-Leu enhances activity at concentrations below 20 mM Homo sapiens
3.4.13.18 D-Val enhances activity at concentrations below 50 mM Homo sapiens
3.4.13.18 Gly prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated Homo sapiens
3.4.13.18 Ser prolinase activity in erythrocytes from both the normal control and the patients with prolidase (EC 3.4.13.9) deficiency is activated Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.18 L-leucine strong inhibition of prolinase activity against Pro-Gly regardless wether MnCl2 is present or not Homo sapiens
3.4.13.18 L-Val strong inhibition of prolinase activity against Pro-Gly regardless wether MnCl2 is present or not Homo sapiens
3.4.13.18 Mn2+ 0.1 mM, inhibits prolinase activity in normal erythrocytes against Pro-Ala, Pro-Val, Pro-Met, Pro-Asp Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.9 3.2
-
 L-Pro-L-Val pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 4.2
-
 L-Pro-L-Met pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 5
-
 L-Pro-L-Met pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 5.5
-
 L-Pro-L-Val pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 6.8
-
 L-Pro-Gly pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine Homo sapiens
3.4.13.9 7.9
-
 L-Pro-L-Val pH 7.8, 37°C, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 8.1
-
 L-Pro-L-Met pH 7.8, 37°C, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 8.35
-
 L-Pro-L-Met pH 7.8, 37°C, presence of 20 mM glycine Homo sapiens
3.4.13.9 8.6
-
 L-Pro-Gly pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 9.7
-
 L-Pro-Gly pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 9.7
-
 L-Pro-L-Met pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 9.9
-
 L-Pro-L-Met pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine Homo sapiens
3.4.13.9 10.1
-
 L-Pro-L-Val pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 13.8
-
 L-Pro-Gly pH 7.8, 37°C, presence of 0.1 mM MnCl2 Homo sapiens
3.4.13.9 14.7
-
 L-Pro-L-Val pH 7.8, 37°C, presence of 20 mM glycine Homo sapiens
3.4.13.9 15.1
-
 L-Pro-Gly pH 7.8, 37°C, presence of 20 mM glycine Homo sapiens
3.4.13.9 16.7
-
 L-Pro-Gly pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 17.3
-
 L-Pro-L-Val pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine Homo sapiens
3.4.13.9 17.6
-
 L-Pro-L-Met pH 7.8, 37°C Homo sapiens
3.4.13.9 18.2
-
 L-Pro-Gly pH 7.8, 37°C, enzyme from patient with enzyme deficiency Homo sapiens
3.4.13.9 19.1
-
 L-Pro-L-Met pH 7.8, 37°C, presence of 0.1 mM MnCl2 Homo sapiens
3.4.13.9 26
-
 L-Pro-L-Val pH 7.8, 37°C Homo sapiens
3.4.13.9 26.9
-
 L-Pro-Gly pH 7.8, 37°C Homo sapiens
3.4.13.9 28.6
-
 L-Pro-L-Val pH 7.8, 37°C, presence of 0.1 mM MnCl2 Homo sapiens
3.4.13.18 3.2
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly Homo sapiens
3.4.13.18 4.2
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly Homo sapiens
3.4.13.18 5
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
3.4.13.18 5.5
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
3.4.13.18 6.8
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
3.4.13.18 7.9
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound Homo sapiens
3.4.13.18 8.1
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound Homo sapiens
3.4.13.18 8.35
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 20 mM Gly Homo sapiens
3.4.13.18 8.6
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
3.4.13.18 9.7
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 Homo sapiens
3.4.13.18 9.7
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 Homo sapiens
3.4.13.18 9.9
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
3.4.13.18 10.1
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 0.1 mM MnCl2 Homo sapiens
3.4.13.18 13.8
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 Homo sapiens
3.4.13.18 14.7
-
 Pro-Val pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 20 mM Gly Homo sapiens
3.4.13.18 15.1
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, activated by 20 mM Gly Homo sapiens
3.4.13.18 16.7
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, activated by 20 mM Gly Homo sapiens
3.4.13.18 17.3
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 and 20 mM Gly Homo sapiens
3.4.13.18 17.6
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound Homo sapiens
3.4.13.18 18.2
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from patients with prolidase (EC 3.4.13.9) deficiency, without activating compound Homo sapiens
3.4.13.18 19.1
-
 Pro-Met pH 7.8, 37°C, erythrocyte lysate from healthy patients, activated by 0.1 mM MnCl2 Homo sapiens
3.4.13.18 26
-
 Pro-Val pH 7.8, 37°C, erythrocyte lysate from healthy patients, without activating compound Homo sapiens
3.4.13.18 26.9
-
 Pro-Gly pH 7.8, 37°, erythrocyte lysate from healthy patients, without activating compound Homo sapiens
3.4.13.18 28.6
-
 Pro-Val pH 7.8, 37°C, erythrocxyte lysate from healthy patients, activated by 0.1 mM MnCl2 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.18 Mn2+ 0.1 mM, increases prolinase activity in normal erythrocytes against Pro-Gly, Pro-Glu, Pro-Leu, Pro-Ser and Pro-Phe Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Homo sapiens
-
 comparison of normal human and a patient with prolidase deficiency
-
3.4.13.18 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.18 erythrocyte lysate Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 L-Pro-Gly + H2O
-
 Homo sapiens L-Pro + Gly
-
 ?
3.4.13.9 L-Pro-L-Ala + H2O
-
 Homo sapiens L-Pro + L-Ala
-
 ?
3.4.13.9 L-Pro-L-Asp + H2O
-
 Homo sapiens L-Pro + L-Asp
-
 ?
3.4.13.9 L-Pro-L-Glu + H2O
-
 Homo sapiens L-Pro + L-Glu
-
 ?
3.4.13.9 L-Pro-L-Leu + H2O
-
 Homo sapiens L-Pro + L-Leu
-
 ?
3.4.13.9 L-Pro-L-Met + H2O
-
 Homo sapiens L-Pro + L-Met
-
 ?
3.4.13.9 L-Pro-L-Phe + H2O
-
 Homo sapiens L-Pro + L-Phe
-
 ?
3.4.13.9 L-Pro-L-Ser + H2O
-
 Homo sapiens L-Pro + L-Ser
-
 ?
3.4.13.9 L-Pro-L-Val + H2O
-
 Homo sapiens L-Pro + L-Val
-
 ?
3.4.13.18 Pro-Ala + H2O
-
 Homo sapiens Pro + Ala
-
 ?
3.4.13.18 Pro-Asp + H2O
-
 Homo sapiens Pro + Asp
-
 ?
3.4.13.18 Pro-Glu + H2O
-
 Homo sapiens Pro + Glu
-
 ?
3.4.13.18 Pro-Gly + H2O
-
 Homo sapiens Pro + Gly
-
 ?
3.4.13.18 Pro-Leu + H2O
-
 Homo sapiens Pro + Leu
-
 ?
3.4.13.18 Pro-Met + H2O
-
 Homo sapiens Pro + Met
-
 ?
3.4.13.18 Pro-Phe + H2O
-
 Homo sapiens Pro + Phe
-
 ?
3.4.13.18 Pro-Ser + H2O
-
 Homo sapiens Pro + Ser
-
 ?
3.4.13.18 Pro-Val + H2O
-
 Homo sapiens Pro + Val
-
 ?