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Literature summary extracted from

  • Siddiqui, K.S.; Poljak, A.; Cavicchioli, R.
    Improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives (2004), Cell. Mol. Biol., 50, 657-667.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.1 biotechnology the chemical modification by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives can be utilized to increase the thermostability of psychrophilic and mesophilic enzymes while retaining their high intrinsic activity or increasinf their activity Pandalus borealis
3.1.3.1 biotechnology the chemical modification by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives can be utilized to increase the thermostability of psychrophilic and mesophilic enzymes while retaining their high intrinsic activity or increasing their activity Bos taurus

General Stability

EC Number General Stability Organism
3.1.3.1 improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives Bos taurus
3.1.3.1 improved activity and stability of alkaline phosphatases from psychrophilic and mesophilic organisms by chemically modifying aliphatic or amino groups using tetracarboxy-benzophenone derivatives Pandalus borealis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.06
-
 4-nitrophenyl phosphate 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
3.1.3.1 0.11
-
 4-nitrophenyl phosphate 37°C, native enzyme Bos taurus
3.1.3.1 0.12
-
 4-nitrophenyl phosphate 37°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
3.1.3.1 0.13
-
 4-nitrophenyl phosphate 37°C, native enzyme Pandalus borealis
3.1.3.1 0.17
-
 4-nitrophenyl phosphate 57°C, native enzyme Bos taurus
3.1.3.1 0.18
-
 4-nitrophenyl phosphate 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 0.2
-
 4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 0.23
-
 4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 0.26
-
 4-nitrophenyl phosphate 10°C, native enzyme Pandalus borealis
3.1.3.1 0.85
-
 4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Bos taurus
-
-
-
3.1.3.1 Pandalus borealis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Bos taurus 4-nitrophenol + phosphate
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Pandalus borealis 4-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 CAP
-
 Bos taurus
3.1.3.1 SAP
-
 Pandalus borealis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 40
-
 native enzyme and enzymes with chemically modified aliphatic or amino groups using tetracarboxy-benzophenone derivatives Pandalus borealis
3.1.3.1 56
-
 native enzyme and enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 58
-
 enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 45
-
 t1/2: 23 min, native enzyme Pandalus borealis
3.1.3.1 50
-
 t1/2: 182 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
3.1.3.1 50
-
 t1/2: 38 min, native enzyme Bos taurus
3.1.3.1 50
-
 t1/2: 58 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 50
-
 t1/2: 77 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 53
-
 t1/2: 15 min, native enzyme Bos taurus
3.1.3.1 53
-
 t1/2: 20 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 53
-
 t1/2: 26 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 55
-
 t1/2: 2.75 min, native enzyme Pandalus borealis
3.1.3.1 55
-
 t1/2: 41 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
3.1.3.1 56
-
 t1/2: 10 min, native enzyme Bos taurus
3.1.3.1 56
-
 t1/2: 12 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 56
-
 t1/2: 14 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 58
-
 t1/2: 1 min, native enzyme Pandalus borealis
3.1.3.1 58
-
 t1/2: 80 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 58
-
 t1/2: 84 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 59
-
 t1/2: 6 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 59
-
 t1/2: 6 min, native enzyme Bos taurus
3.1.3.1 59
-
 t1/2: 7 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 60
-
 t1/2: 0.7 min, native enzyme Pandalus borealis
3.1.3.1 60
-
 t1/2: 10 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
3.1.3.1 62
-
 t1/2: 35 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 62
-
 t1/2: 37 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 65
-
 t1/2: 1 min, native enzyme Bos taurus
3.1.3.1 65
-
 t1/2: 2.6 min, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction (30 min, 15 mM) Pandalus borealis
3.1.3.1 65
-
 t1/2: 24 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 65
-
 t1/2: 25 min, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 69
-
 t1/2: 6 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 73
-
 t1/2: 4 min, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.1 0.037 0.23 4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 0.55
-
 4-nitrophenyl phosphate 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
3.1.3.1 14.67
-
 4-nitrophenyl phosphate 10°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
3.1.3.1 20 50 4-nitrophenyl phosphate 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 65
-
 4-nitrophenyl phosphate 37°C, native enzyme Bos taurus
3.1.3.1 77.4
-
 4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 78.83
-
 4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 87.83
-
 4-nitrophenyl phosphate 57°C, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Bos taurus
3.1.3.1 115.9
-
 4-nitrophenyl phosphate 37°C, native enzyme Bos taurus
3.1.3.1 153.9
-
 4-nitrophenyl phosphate 37°C, enzyme with aliphatic-group modification by 3,3',4,4'-tetracarboxylic acid in a photo-assisted reaction Pandalus borealis
3.1.3.1 155.7
-
 4-nitrophenyl phosphate 10°C, native enzyme Pandalus borealis
3.1.3.1 183.3
-
 4-nitrophenyl phosphate 57°C, native enzyme Bos taurus
3.1.3.1 812.3
-
 4-nitrophenyl phosphate 37°C, native enzyme Pandalus borealis
3.1.3.1 1443
-
 4-nitrophenyl phosphate 37°C, enzyme with amino-group modification in the dark, pH 7, with 12.5 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis
3.1.3.1 16230
-
 4-nitrophenyl phosphate 37°C, native enzyme, enzyme with amino-group modification in the dark, pH 8, with 6.25 mM 3,3',4,4'-benzophenone tetracarboxylic dianhydride Pandalus borealis