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Literature summary extracted from

  • Win, T.T.; Isono, N.; Kusnadi, Y.; Watanabe, K.; Obae, K.; Ito, H.; Matsui, H.
    Enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with beta-fructofuranosidase from Arthrobacter globiformis (2004), Biotechnol. Lett., 26, 499-503.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 2.4
-
 sucrose pH 7.0, 37°C Arthrobacter globiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 60000
-
 x * 60000, native enzyme, SDS-PAGE Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Arthrobacter globiformis
-
-
-
3.2.1.26 Arthrobacter globiformis IFO 3062
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 native enzyme 37fold by ammonium sulfate fractionation, dialysis, and two steps of ion exchange chromatography Arthrobacter globiformis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.26 raffinose + H2O = beta-D-fructofuranose + 6-O alpha-D-galactopyranosyl-alpha-D-glucopyranose the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism Arthrobacter globiformis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 101
-
 purified enzyme Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 additional information the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview Arthrobacter globiformis ?
-
 ?
3.2.1.26 additional information the enzyme shows also transfructosylation activity with cellobiose or cellotriose as acceptor and sucrose as donor, mechanism, overview Arthrobacter globiformis IFO 3062 ?
-
 ?
3.2.1.26 sucrose + H2O
-
 Arthrobacter globiformis D-glucose + D-fructose
-
 ?
3.2.1.26 sucrose + H2O
-
 Arthrobacter globiformis IFO 3062 D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 ? x * 60000, native enzyme, SDS-PAGE Arthrobacter globiformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 beta-FFase
-
 Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 37
-
-
 Arthrobacter globiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 40
-
 15 min, purified enzyme, over 90% remaining activity Arthrobacter globiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 127
-
 sucrose pH 7.0, 37°C Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 6.8
-
-
 Arthrobacter globiformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 5.5 9.5 purified enzyme, over 90% of activity after 20 h at 4°C Arthrobacter globiformis