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Literature summary extracted from

  • Akao, T.; Yamaguchi, M.; Yahara, A.; Yoshiuchi, K.; Fujita, H.; Yamada, O.; Akita, O.; Ohmachi, T.; Asada, Y.; Yoshida, T.
    Cloning and expression of 1,2-alpha-mannosidase gene (fmanIB) from filamentous fungus Aspergillus oryzae: in vivo visualization of the FmanIBp-GFP fusion protein (2006), Biosci. Biotechnol. Biochem., 70, 471-479.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.113 expression by an Aspergillus host Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin 0.2 mM, 50% inhibition Aspergillus oryzae
3.2.1.113 EDTA enzyme loses 96% activity after dialysis in 50 mM PIPES buffer, pH 6.0, containing 2 mM EDTA. Addition of 10 mM Ca2+ recovers activity to 100%, addition of 10 mM Mg2+ recovers activity to 43% Aspergillus oryzae
3.2.1.113 kifunensine 0.0015 mM, 50% inhibition Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.113 extracellular with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1 Aspergillus oryzae
-
-
3.2.1.113 intracellular with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1 Aspergillus oryzae 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.113 Ca2+ 10 mM, completely recovers activity after treatment with EDTA Aspergillus oryzae
3.2.1.113 Mg2+ 10 mM, recovers activity to 43% after treatment with EDTA Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.113 67000
-
 x * 67000, SDS-PAGE Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.113 glycoprotein
-
 Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113
-
 Aspergillus oryzae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.113 culture supernatant
-
 Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 additional information
-
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 1,2-alpha-D-mannobiose + H2O
-
 Aspergillus oryzae D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Aspergillus oryzae (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 additional information no activity with p-nitrophenyl-alpha-mannoside, Manalpha(1-3)Man-OMe or Manalpha(1-6)Man-OMe Aspergillus oryzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 ? x * 67000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.113 FmanIBp
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 45
-
 hydrolysis of 1,2-alpha-mannobiose Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.113 45 60 45°C: optimum, 60°C: 60% of maximal activity, 65°C: 14% of maximal activity, hydrolysis of 1,2-alpha-mannobiose Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 5.5
-
 hydrolysis of 1,2-alpha-mannobiose Aspergillus oryzae