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Literature summary extracted from

  • Woo, K.K.; Kimura, Y.
    Regulation of substrate specificity of plant alpha-mannosidase by cobalt ion: in vitro hydrolysis of high-mannose type N-glycans by Co2+-activated Ginkgo alpha-mannosidase (2005), Biosci. Biotechnol. Biochem., 69, 1111-1119.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.114 Co2+ activates, responsible for regulation of substrate specificity Ginkgo biloba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.114 120000
-
 x * 120000, SDS-PAGE Ginkgo biloba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.114 additional information Ginkgo biloba Co2+ regulates the substrate specificity, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Ginkgo biloba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.114 native enzyme from mature seeds to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration Ginkgo biloba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.114 seed
-
 Ginkgo biloba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 4-nitrophenyl-alpha-D-mannoside + H2O
-
 Ginkgo biloba 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.114 Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
3.2.1.114 Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
3.2.1.114 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
3.2.1.114 Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
-
 Ginkgo biloba Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
-
 ?
3.2.1.114 additional information Co2+ regulates the substrate specificity, overview Ginkgo biloba ?
-
 ?
3.2.1.114 additional information in vitro hydrolysis of high-mannose type N-glycans, removal of terminal alpha-1,2-mannose residues, substrate specificity and isomeric substrate/product structures, overview Ginkgo biloba ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.114 ? x * 120000, SDS-PAGE Ginkgo biloba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.114 37
-
 assay at Ginkgo biloba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.114 5
-
-
 Ginkgo biloba