Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tomatsu, M.; Kondo, T.; Yoshikawa, T.; Komeno, T.; Adachi, N.; Kawasaki, Y.; Ikuta, A.; Tashiro, F.
    An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata (2004), Biol. Chem., 385, 819-827.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.22 additional information the cytotoxic enzyme induces apoptosis in transformed cells Aralia elata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.22 fucose glucose, mannose, and N-acetylglucosamine show poor inhibitory effects Aralia elata
3.2.2.22 galactose and derivatives, inhibits the incorporation of the enzyme into target cells via its cell surface receptor, and inhibits the inhibition of protein synthesis through the enzyme, minimum inhibition concentration is 3.3 mM Aralia elata
3.2.2.22 lactose minimum inhibition concentration is 0.8 mM Aralia elata
3.2.2.22 melibiose minimum inhibition concentration is 1.0 mM Aralia elata
3.2.2.22 N-acetylgalactosamine minimum inhibition concentration is 4.2 mM Aralia elata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.22 extracellular the enzyme enters target cells via its cell surface receptor Aralia elata
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.22 29000
-
 1 * 29000, A-chain, + 1 * 32000, B-chain, SDS-PAGE Aralia elata
3.2.2.22 32000
-
 1 * 29000, A-chain, + 1 * 32000, B-chain, SDS-PAGE Aralia elata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.22 additional information Aralia elata the cytotoxic enzyme induces apoptosis in transformed cells by inhibiting protein synthesis and inactivating ribosomes, the enzyme targets diverse cells, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.22 Aralia elata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.2.22 glycoprotein
-
 Aralia elata

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.22 native enzyme 611.4fold from shoots by ammonium sulfate fractionation, ion exchange chromatography and gel filtration Aralia elata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.22 shoot
-
 Aralia elata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.22 additional information
-
 cytotoxic effects on WI-38 and VA-13 cells Aralia elata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.22 28S rRNA + H2O substrate from rat liver ribosomes, generation of an R-fragment from 28S RNA, product determination using aniline coupling Aralia elata ?
-
 ?
3.2.2.22 additional information the cytotoxic enzyme induces apoptosis in transformed cells by inhibiting protein synthesis and inactivating ribosomes, the enzyme targets diverse cells, overview Aralia elata ?
-
 ?
3.2.2.22 additional information the enzyme targets diverse cells, e.g. WI-38 cells or VA-13 cells, overview Aralia elata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.22 dimer 1 * 29000, A-chain, + 1 * 32000, B-chain, SDS-PAGE Aralia elata

Synonyms

EC Number Synonyms Comment Organism
3.2.2.22 aralin
-
 Aralia elata
3.2.2.22 RIP
-
 Aralia elata
3.2.2.22 RNA N-glycosidase
-
 Aralia elata
3.2.2.22 type II ribosome-inactivating protein
-
 Aralia elata