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Literature summary extracted from

  • Wang, L.C.; Kang, L.; Hu, T.M.; Wang, J.L.
    Abrin-a A chain expressed as soluble form in Escherichia coli from a PCR-synthesized gene is catalytically and functionally active (2004), Biochimie, 86, 327-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.22 expression of His6-tagged optimized abricin A-chain in Escherichia coli strain BL21(DE3) Abrus precatorius

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.22 additional information optimization of the abricin A-chain by replacing rare codons with high-frequency ones, overview Abrus precatorius

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.22 SDS inhibition at 0.5% Abrus precatorius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.22 extracellular the enzyme enters target cells via its cell surface receptor Abrus precatorius
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.22 30000
-
 2 * 30000, recombinant abricin A-chain, SDS-PAGE Abrus precatorius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.22 additional information Abrus precatorius the cytotoxic enzyme irreversibly inactivates eukaryotic ribosomes in target cells, e.g. Sp2/0 cells and SMMC-7721 cells, by depurinating a key adenine residue from a highly conserved GAGA loop in 28S rRNA thereby inhibiting protein synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.22 Abrus precatorius Q7DM12
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.22 recombinant His6-tagged optimized abricin A-chain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Abrus precatorius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.22 seed
-
 Abrus precatorius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.22 additional information
-
 50% inhibition of L-leucine incorporation in a protein inhibition assay in a rabbit reticulocyte cell-free system Abrus precatorius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.22 28S RNA + H2O substrate from rat liver ribosomes, depurination of A4324, generation of a 420nt fragment, product determination using aniline coupling Abrus precatorius ?
-
 ?
3.2.2.22 additional information the cytotoxic enzyme irreversibly inactivates eukaryotic ribosomes in target cells, e.g. Sp2/0 cells and SMMC-7721 cells, by depurinating a key adenine residue from a highly conserved GAGA loop in 28S rRNA thereby inhibiting protein synthesis Abrus precatorius ?
-
 ?
3.2.2.22 additional information protein inhibition assay in a rabbit reticulocyte cell-free system with inhibition of L-leucine incorporation Abrus precatorius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.22 dimer 2 * 30000, recombinant abricin A-chain, SDS-PAGE Abrus precatorius
3.2.2.22 More the A-chain exhibits the RNA N-glycosidase activity, while the B-chain mediates cell entry by binding to the target cell surface glycolipid receptor Abrus precatorius

Synonyms

EC Number Synonyms Comment Organism
3.2.2.22 ABRaA
-
 Abrus precatorius
3.2.2.22 abrin
-
 Abrus precatorius
3.2.2.22 abrin-a A chain
-
 Abrus precatorius
3.2.2.22 More the enzyme belongs to the type-II-ribosome-inactivating protein family Abrus precatorius
3.2.2.22 RNA N-glycosidase
-
 Abrus precatorius
3.2.2.22 type II ribosome-inactivating protein
-
 Abrus precatorius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.22 37
-
 assay at Abrus precatorius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.22 7.6
-
 assay at Abrus precatorius