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Literature summary extracted from

  • Milad-Kodsi, E.; Langston, T.B.; Gergis, M.R.; Grogan, W.M.
    Acidic residues emulate a phosphorylation switch to enhance the activity of rat hepatic neutral cytosolic cholesterol esterase (2005), Biochim. Biophys. Acta, 1734, 62-73.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.13 G505D/S506D 18fold increase in activity against cholesterol oleate, decrease in activity against p-nitrophenyl octanoate Rattus norvegicus
3.1.1.13 G505N/S506N/K496Q/R503Q no activity with cholesterol oleate, reduced activity with p-nitrophenyl substrates Rattus norvegicus
3.1.1.13 G505N/S506N/R503Q no activity Rattus norvegicus
3.1.1.13 K496Q/R503Q no activity with cholesterol oleate, or p-nitrophenyl octanoate, reduced activity with p-nitrophenyl acetate Rattus norvegicus
3.1.1.13 additional information mutation S506D plus insertion of D in position 507, no activity with cholesterol oleate, reduced activity with p-nitrophenyl substrates Rattus norvegicus
3.1.1.13 S506D 50% decrease in activity against cholesterol oleate, increase in activity against p-nitrophenyl octanoate, nitrophenyl acetate Rattus norvegicus
3.1.1.13 S506K no activity with cholesterol oleate, somehow reduced activity with p-nitrophenyl substrates Rattus norvegicus
3.1.1.13 S506N no activity with cholesterol oleate, somehow reduced activity with p-nitrophenyl substrates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.13 0.144
-
 p-nitrophenyl octanoate mutant G505D/S506D, pH 7.4, 37°C Rattus norvegicus
3.1.1.13 0.316
-
 p-nitrophenyl octanoate mutant S506D, pH 7.4, 37°C Rattus norvegicus
3.1.1.13 1.639
-
 p-nitrophenyl octanoate mutant S506N, pH 7.4, 37°C Rattus norvegicus
3.1.1.13 1.782
-
 p-nitrophenyl octanoate mutant S506K, pH 7.4, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.13 cytosol
-
 Rattus norvegicus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.13 phosphoprotein different activity against p-nitrophenyl and cholesteryl substrates is influenced by phosphorylation state of S506 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl oleate + H2O
-
 Rattus norvegicus cholesterol + oleic acid
-
 ?
3.1.1.13 nitrophenyl acetate + H2O
-
 Rattus norvegicus p-nitrophenol + acetate
-
 ?
3.1.1.13 p-nitrophenyl octanoate + H2O
-
 Rattus norvegicus p-nitrophenol + octanoic acid
-
 ?