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Literature summary extracted from

  • Zouari, N.; Miled, N.; Cherif, S.; Mejdoub, H.; Gargouri, Y.
    Purification and characterization of a novel lipase from the digestive glands of a primitive animal: The scorpion (2005), Biochim. Biophys. Acta, 1726, 67-74.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 additional information
-
 additional information kinetics Scorpio maurus
3.1.1.3 17
-
 tributyrin pH 9.0, 37°C Scorpio maurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information the enzyme contains a single SH group accessible to sulfhydryl reagents and not essential for activity Scorpio maurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 50000
-
 gel filtration Scorpio maurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Scorpio maurus
-
 collected in Tunisia
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein sugar content about 15% of the molecular mass Scorpio maurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native enzyme 506fold from scorpion digestive glands by anion exchange chromatography, ammonium sulfate fractionation, gel filtration, and cation exchange chromatography to homogeneity Scorpio maurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate the enzyme contains an active site serine residue Scorpio maurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 digestive gland
-
 Scorpio maurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
 titrimetric assay method Scorpio maurus
3.1.1.3 5023
-
 purified enzyme Scorpio maurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 tributyrin + H2O
-
 Scorpio maurus dibutyrin + butyrate
-
 ?
3.1.1.3 triolein + H2O olive oil Scorpio maurus diolein + oleate
-
 ?
3.1.1.3 tripropionin + H2O
-
 Scorpio maurus dipropionin + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 50000, SDS-PAGE Scorpio maurus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Scorpio maurus
3.1.1.3 scorpio digestive lipase
-
 Scorpio maurus
3.1.1.3 SDL
-
 Scorpio maurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
-
 Scorpio maurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 55
-
 inactivation above within 5 min Scorpio maurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.3 5370
-
 tributyrin pH 9.0, 37°C Scorpio maurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9
-
-
 Scorpio maurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5 11 inactive below pH 5.0 Scorpio maurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 11
-
 4 h, 65% remaining activity Scorpio maurus