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Literature summary extracted from

  • Kurakake, M.; Fujii, T.; Yata, M.; Okazaki, T.; Komaki, T.
    Characteristics of transxylosylation by beta-xylosidase from Aspergillus awamori K4 (2005), Biochim. Biophys. Acta, 1726, 272-279.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 gene Xaw1, DNA and amino acid sequence determination and analysis Aspergillus awamori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 87156
-
x * 87156, sequence calculation, x * 115000, SDS-PAGE Aspergillus awamori
3.2.1.37 115000
-
x * 87156, sequence calculation, x * 115000, SDS-PAGE Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Aspergillus awamori Q4AEG8 gene Xaw1
-
3.2.1.37 Aspergillus awamori K4 Q4AEG8 gene Xaw1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein
-
Aspergillus awamori

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 native enzyme by acetone precipitation, freeze-drying, gel filtration, anion exchange chromatography, and a second step of gel filtration Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
Aspergillus awamori 4-nitrophenol + beta-D-xylose
-
?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
Aspergillus awamori K4 4-nitrophenol + beta-D-xylose
-
?
3.2.1.37 additional information the enzyme also performs transxylosylation reactions producing xylopentaose, xylotetraose, and xylotriose from xylobiose and xylose, the enzyme is active with various acceptors, e.g. alcohols, sugar alcohols, phenols, hydroxybenzylalcohols, and disaccharides, detailed overview Aspergillus awamori ?
-
?
3.2.1.37 additional information the enzyme also performs transxylosylation reactions producing xylopentaose, xylotetraose, and xylotriose from xylobiose and xylose, the enzyme is active with various acceptors, e.g. alcohols, sugar alcohols, phenols, hydroxybenzylalcohols, and disaccharides, detailed overview Aspergillus awamori K4 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 87156, sequence calculation, x * 115000, SDS-PAGE Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 beta-xylosidase
-
Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 40
-
assay at Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5
-
assay at Aspergillus awamori