Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chessa, J.P.; Petrescu, I.; Bentahir, M.; Van Beeumen, J.; Gerday, C.
    Purification, physico-chemical characterization and sequence of a heat labile alkaline metalloprotease isolated from a psychrophilic Pseudomonas species (2000), Biochim. Biophys. Acta, 1479, 265-274.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.40
-
 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 1,10-phenanthroline
-
 Pseudomonas sp.
3.4.24.40 EDTA 10 mM, pH 8, strong inhibition Pseudomonas sp.
3.4.24.40 EGTA 10 mM, pH 8, strong inhibition Pseudomonas sp.
3.4.24.40 HgCl2 complete inhibition Pseudomonas sp.
3.4.24.40 additional information not inhibited by dithiothreitol, PMSF and DFP Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.40 extracellular
-
 Pseudomonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Ca2+ stabilizing Pseudomonas sp.
3.4.24.40 Zn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 48690
-
 electrospray mass spectrometry Pseudomonas sp.
3.4.24.40 48690
-
 calculated Pseudomonas sp.
3.4.24.40 48730
-
 electrospray mass spectrometry Pseudomonas sp.
3.4.24.40 48770
-
 electrospray mass spectrometry Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Pseudomonas sp.
-
-
-
3.4.24.40 Pseudomonas sp. TAC-II-18
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.40 additional information
-
 113 U/mg, one unit gives an increase of 0.01 absorbance at 366 nm, excess of azocasein in 20 mM Tris, 1 mM CaCl2, pH 8 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 azocasein + H2O
-
 Pseudomonas sp. ?
-
 ?
3.4.24.40 azocasein + H2O
-
 Pseudomonas sp. TAC-II-18 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.40 psychrophilic alkaline metalloprotease PAP Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.40 4
-
 highest acitivity after 4 days of culture Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 45
-
 50% activity after 20 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 9 10
-
 Pseudomonas sp.