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Literature summary extracted from

  • Bukhtojarov, F.E.; Ustinov, B.B.; Salanovich, T.N.; Antonov, A.I.; Gusakov, A.V.; Okunev, O.N.; Sinitsyn, A.P.
    Cellulase complex of the fungus Chrysosporium lucknowense: isolation and characterization of endoglucanases and cellobiohydrolases (2004), Biochemistry, 69, 542-551.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information KM-value for carboxymethyl cellulase is 1.3 g/l, KM-value for beta-glucan is 1.14 g/l, endoglucanase EG44 Chrysosporium lucknowense
3.2.1.4 additional information
-
 additional information KM-value for carboxymethyl cellulase is 11 g/l, KM-value for beta-glucan is 0.12 g/l, endoglucanase EG28 Chrysosporium lucknowense
3.2.1.4 additional information
-
 additional information KM-value for carboxymethyl cellulase is 5.4 g/l, KM-value for beta-glucan is 3.0 g/l, endoglucanase EG25 Chrysosporium lucknowense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 25000
-
 x * 25000, endoglucanase EG25, SDS-PAGE Chrysosporium lucknowense
3.2.1.4 28000
-
 x * 28000, endoglucanase EG28, SDS-PAGE Chrysosporium lucknowense
3.2.1.4 44000
-
 x * 44000, endoglucanase EG44, SDS-PAGE Chrysosporium lucknowense

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Chrysosporium lucknowense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 endoglucanase EG25 Chrysosporium lucknowense
3.2.1.4 endoglucanase EG28 Chrysosporium lucknowense
3.2.1.4 endoglucanase EG44 Chrysosporium lucknowense

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 culture medium
-
 Chrysosporium lucknowense
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O endoglucanase EG25 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 beta-glucan + H2O endoglucanase EG28 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 beta-glucan + H2O endoglucanase EG44 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG25 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG28 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O endoglucanase EG44 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG25 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG28 Chrysosporium lucknowense ?
-
 ?
3.2.1.4 p-nitrophenyl-beta-D-cellobioside + H2O endoglucanase EG44 Chrysosporium lucknowense ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 25000, endoglucanase EG25, SDS-PAGE Chrysosporium lucknowense
3.2.1.4 ? x * 28000, endoglucanase EG28, SDS-PAGE Chrysosporium lucknowense
3.2.1.4 ? x * 44000, endoglucanase EG44, SDS-PAGE Chrysosporium lucknowense

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EG25
-
 Chrysosporium lucknowense
3.2.1.4 EG28
-
 Chrysosporium lucknowense
3.2.1.4 EG44 EG44 and RG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module Chrysosporium lucknowense
3.2.1.4 endoglucanase EG25
-
 Chrysosporium lucknowense
3.2.1.4 endoglucanase EG28
-
 Chrysosporium lucknowense
3.2.1.4 endoglucanase EG44 endoglucanase EG44 and endoglucanase EG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module Chrysosporium lucknowense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
 endoglucanase EG28 Chrysosporium lucknowense
3.2.1.4 65
-
 endoglucanase EG25 Chrysosporium lucknowense
3.2.1.4 70
-
 endoglucanase EG44 Chrysosporium lucknowense

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 30 75 30°C: about 55% of maximal activity, 75°C: about 60% of maximal activity, endoglucanase EG28 Chrysosporium lucknowense
3.2.1.4 45 75 45°C: about 45% of maximal activity, 75°C: about 60% of maximal activity, endoglucanase EG25 Chrysosporium lucknowense
3.2.1.4 50 75 50°C: about 35% of maximal activity, 75°C: about 60% of maximal activity, endoglucanase EG44 Chrysosporium lucknowense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 3.8
-
 beta-Glucan 50°C, pH 5.5, endoglucanase EG25 Chrysosporium lucknowense
3.2.1.4 15
-
 carboxymethyl cellulose 50°C, pH 5.5, endoglucanase EG25 Chrysosporium lucknowense
3.2.1.4 19
-
 carboxymethyl cellulose 50°C, pH 5.5, endoglucanase EG28 Chrysosporium lucknowense
3.2.1.4 54
-
 carboxymethyl cellulose 50°C, pH 5.5, endoglucanase EG44 Chrysosporium lucknowense
3.2.1.4 64
-
 beta-Glucan 50°C, pH 5.5, endoglucanase EG28 Chrysosporium lucknowense
3.2.1.4 76
-
 beta-Glucan 50°C, pH 5.5, endoglucanase EG44 Chrysosporium lucknowense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
-
 Chrysosporium lucknowense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3.5 8.5 pH 3.5: about 50% of maximal activity, pH 8.5: about 55% of maximal activity, endoglucanase EG25 Chrysosporium lucknowense
3.2.1.4 4 7.5 pH 4.0: about 60% of maximal activity, pH 7.5: about 35% of maximal activity, endoglucanase EG28 Chrysosporium lucknowense
3.2.1.4 4 7.5 pH 4.0: about 75% of maximal activity, pH 7.5: about 65% of maximal activity, endoglucanase EG44 Chrysosporium lucknowense

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 Chrysosporium lucknowense isoelectric focusing, endoglucanase EG25
-
 4
3.2.1.4 Chrysosporium lucknowense isoelectric focusing, endoglucanase EG28
-
 5.7
3.2.1.4 Chrysosporium lucknowense isoelectric focusing, endoglucanase EG44
-
 6