EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.184 | gene rhlI, overexpression in Escherichia coli strain XL 1-Blue as maltose binding protein-fusion protein, overexpression of His-tagged RhlI in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.184 | 0.0059 | - |
butyryl-[acyl-carrier protein 2] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
2.3.1.184 | 0.0074 | - |
butyryl-[acyl-carrier protein 1] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
2.3.1.184 | 0.2 | - |
butyryl-CoA | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
2.3.1.184 | 0.283 | - |
butyryl-[acyl-carrier protein 3] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.184 | Mg2+ | - |
Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.184 | S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | Pseudomonas aeruginosa | substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.184 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.184 | recombinant maltose binding protein-fusion enzyme RhlI from Escherichia coli strain XL 1-Blue by amylose affinity chromatography, recombinant His-tagged RhlI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.184 | an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone | structures of substrate, product, and transition state, overview | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.184 | S-adenosyl-L-methionine + butyryl-CoA | substrate of RhlI, very low activity | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA | - |
? | |
2.3.1.184 | S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 1] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 1] | - |
? | |
2.3.1.184 | S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 2] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 2] | - |
? | |
2.3.1.184 | S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 3] | substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates, very low activity with ACP3 | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 3] | - |
? | |
2.3.1.184 | S-adenosyl-L-methionine + butyryl-[acyl-carrier protein] | substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity | Pseudomonas aeruginosa | 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.184 | acylhomoserine lactone synthase | - |
Pseudomonas aeruginosa |
2.3.1.184 | RhlI | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.184 | 25 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.184 | 0.026 | - |
butyryl-[acyl-carrier protein 3] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
2.3.1.184 | 0.05 | - |
butyryl-CoA | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
2.3.1.184 | 0.35 | - |
butyryl-[acyl-carrier protein 1] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa | |
2.3.1.184 | 0.46 | - |
butyryl-[acyl-carrier protein 2] | pH 8.8, 25°C, recombinant enzyme | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.184 | 8.8 | - |
assay at | Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.184 | additional information | - |
pH-profile | Pseudomonas aeruginosa |