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Literature summary extracted from

  • Kaplan, D.; Barak, D.; Ordentlich, A.; Kronman, C.; Velan, B.; Shafferman, A.
    Is aromaticity essential for trapping the catalytic histidine 447 in human acetylcholinesterase? (2004), Biochemistry, 43, 3129-3136.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 F295A KM-value similar to wild-type Homo sapiens
3.1.1.7 F295A/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295D strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295D/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295I/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295L 30% increase in Km-value Homo sapiens
3.1.1.7 F295L/F338A 2fold increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295M KM-value similar to wild-type Homo sapiens
3.1.1.7 F295M/F338A 70% increase in Km-value, 75% decrease in kcat-value Homo sapiens
3.1.1.7 F295N/F338A 6fold increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295Q/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295R strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295R/F338A 8fold increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295S/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295T/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295V/F338A 12fold increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295W/F338A 2fold increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F295Y 60% decrease in Km-value Homo sapiens
3.1.1.7 F295Y/F338A 6fold increase in Km-value, strong decrease in kcat-value Homo sapiens
3.1.1.7 F338A 80% increase in Km-value, 50% decrease in kcat-value Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 diisopropyl phosphorofluoridate
-
 Homo sapiens
3.1.1.7 soman
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.05
-
 acetylthiocholine pH 8.0, 27°C, mutant F295Y Homo sapiens
3.1.1.7 0.13
-
 acetylthiocholine pH 8.0, 27°C, mutant F295A Homo sapiens
3.1.1.7 0.13
-
 acetylthiocholine pH 8.0, 27°C, mutant F295M Homo sapiens
3.1.1.7 0.15
-
 acetylthiocholine pH 8.0, 27°C, wild-type Homo sapiens
3.1.1.7 0.2
-
 acetylthiocholine pH 8.0, 27°C, mutant F295L Homo sapiens
3.1.1.7 0.22
-
 acetylthiocholine pH 8.0, 27°C, mutant F295M/F338A Homo sapiens
3.1.1.7 0.24
-
 acetylthiocholine pH 8.0, 27°C, mutant F338A Homo sapiens
3.1.1.7 0.3
-
 acetylthiocholine pH 8.0, 27°C, mutant F295L/F338A Homo sapiens
3.1.1.7 0.3
-
 acetylthiocholine pH 8.0, 27°C, mutant F295W/F338A Homo sapiens
3.1.1.7 0.8
-
 acetylthiocholine pH 8.0, 27°C, mutant F295Y/F338A Homo sapiens
3.1.1.7 1
-
 acetylthiocholine pH 8.0, 27°C, mutant F295N/F338A Homo sapiens
3.1.1.7 1.2
-
 acetylthiocholine pH 8.0, 27°C, mutant F295R/F338A Homo sapiens
3.1.1.7 1.7
-
 acetylthiocholine pH 8.0, 27°C, mutant F295V/F338A Homo sapiens
3.1.1.7 3
-
 acetylthiocholine pH 8.0, 27°C, mutant F295Q/F338A Homo sapiens
3.1.1.7 3.4
-
 acetylthiocholine pH 8.0, 27°C, mutant F295R Homo sapiens
3.1.1.7 4.1
-
 acetylthiocholine pH 8.0, 27°C, mutant F295D Homo sapiens
3.1.1.7 4.9
-
 acetylthiocholine pH 8.0, 27°C, mutant F295A/F338A Homo sapiens
3.1.1.7 5
-
 acetylthiocholine pH 8.0, 27°C, mutant F295I/F338A Homo sapiens
3.1.1.7 5.2
-
 acetylthiocholine pH 8.0, 27°C, mutant F295S/F338A Homo sapiens
3.1.1.7 5.7
-
 acetylthiocholine pH 8.0, 27°C, mutant F295D/F338A Homo sapiens
3.1.1.7 14
-
 acetylthiocholine pH 8.0, 27°C, mutant F295T/F338A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylthiocholine + H2O
-
 Homo sapiens acetate + thiocholine
-
 ?