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Literature summary extracted from

  • Rudrabhatla, P.; Rajasekharan, R.
    Functional characterization of peanut serine/threonine/tyrosine protein kinase: molecular docking and inhibition kinetics with tyrosine kinase inhibitors (2004), Biochemistry, 43, 12123-12132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.12.1 expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Arachis hypogaea

Protein Variants

EC Number Protein Variants Comment Organism
2.7.12.1 K160R site-directed mutagenesis, inactive mutant enzyme Arachis hypogaea
2.7.12.1 Y148F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea
2.7.12.1 Y213F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea
2.7.12.1 Y297F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea
2.7.12.1 Y317F site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.12.1 genistein competitive inhibition versus ATP, uncompetitive inhibition versus histone H1 Arachis hypogaea
2.7.12.1 additional information molecular docking with tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes Arachis hypogaea
2.7.12.1 staurosporine competitive versus ATP, mixed inhibition versus histone H1 Arachis hypogaea
2.7.12.1 tyrphostin competitive versus ATP, mixed inhibition versus histone H1 Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.12.1 additional information
-
 additional information kinetics Arachis hypogaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.12.1 Mg2+
-
 Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.12.1 55000
-
 recombinant His-tagged wild-type enzyme, gel filtration Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.12.1 additional information Arachis hypogaea the enzyme is developmentally regulated ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.12.1 Arachis hypogaea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.12.1 phosphoprotein the enzyme performs autophosphorylation, mechanism and phosphorylation sites, overview Arachis hypogaea

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.12.1 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.12.1 ATP + histone H1
-
 Arachis hypogaea ADP + phosphorylated histone H1
-
 ?
2.7.12.1 additional information the enzyme is developmentally regulated Arachis hypogaea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.12.1 More structure modeling of wild-type and mutant enzymes based on the X-ray structure of homologous sequences, computational docking and molecular dynamics simulations, overview Arachis hypogaea

Synonyms

EC Number Synonyms Comment Organism
2.7.12.1 serine/threonine/tyrosine protein kinase
-
 Arachis hypogaea
2.7.12.1 STY protein kinase
-
 Arachis hypogaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.12.1 30
-
 assay at Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.12.1 7.5
-
 assay at Arachis hypogaea

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.12.1 ATP
-
 Arachis hypogaea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.12.1 additional information
-
 additional information inhibition kinetics of tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes Arachis hypogaea
2.7.12.1 0.000014
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
2.7.12.1 0.000017
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
2.7.12.1 0.000018
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.00002
-
 genistein versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.000055
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
2.7.12.1 0.000056
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
2.7.12.1 0.000059
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
2.7.12.1 0.000069
-
 staurosporine versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.000112
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
2.7.12.1 0.000186
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea
2.7.12.1 0.000187
-
 tyrphostin versus ATP, pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
2.7.12.1 0.000191
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
2.7.12.1 0.000194
-
 genistein versus ATP, pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea
2.7.12.1 0.000202
-
 tyrphostin versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.000426
-
 genistein versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.000455
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme Arachis hypogaea
2.7.12.1 0.000464
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea
2.7.12.1 0.000549
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y213F Arachis hypogaea
2.7.12.1 0.000571
-
 staurosporine pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
2.7.12.1 0.000599
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y317F Arachis hypogaea
2.7.12.1 0.000621
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y148F Arachis hypogaea
2.7.12.1 0.000694
-
 staurosporine versus histone H1, pH 7.5, 30°C, recombinant mutant Y297F Arachis hypogaea