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Literature summary extracted from

  • Serina, L.; Blondin, C.; Krin, E.; Sismeiro, O.; Danchin, A.; Sakamoto, H.; Gilles, A.M.; Barzu, O.
    Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP (1995), Biochemistry, 34, 5066-5074.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 cGMP lower affinity or extent of activation than GTP Escherichia coli
2.7.4.22 dGTP lower affinity or extent of activation than GTP Escherichia coli
2.7.4.22 dialdehyde-GTP slight stimulatory effect, 30% maximal increase in UMP-kinase activity Escherichia coli
2.7.4.22 GMP lower affinity or extent of activation than GTP Escherichia coli
2.7.4.22 GTP activates only the wild-type, not the mutant D201N Escherichia coli
2.7.4.22 guanosine lower affinity or extent of activation than GTP Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22 overproduced in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.22 D201N exhibits 10% of the wild-type activity, has altered stability and regulatory properties Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 GTP mutant D201N Escherichia coli
2.7.4.22 guanylyl imidodiphosphate mutant D201N Escherichia coli
2.7.4.22 additional information no inhibition by dUMP, CMP, dCMP or TMP Escherichia coli
2.7.4.22 UTP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.05
-
 UMP at 1 mM ATP, wild-type Escherichia coli
2.7.4.22 0.11
-
 UMP mutant D201N Escherichia coli
2.7.4.22 0.12
-
 ATP at 1 mM UMP, wild-type Escherichia coli
2.7.4.22 0.32
-
 ATP mutant D201N Escherichia coli
2.7.4.22 1
-
 GTP wild-type Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 25800
-
 monomer, calculated from amino acid sequence Escherichia coli
2.7.4.22 156000
-
 sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Escherichia coli
-
 ADP + UDP
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Escherichia coli P0A7E9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22 to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 0.13
-
 mutant D201N, rate of the reverse reaction Escherichia coli
2.7.4.22 3.6
-
 mutant D201N, in the presence of 1 mM ATP and 1 mM UMP Escherichia coli
2.7.4.22 160
-
 wild-type, in the presence of ATP and GTP Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.22 room temperature, 0.1 M borate buffer (pH 8.5), 2 months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 r
2.7.4.22 ATP + UMP 30°C, pH 7.4, 2 mM MgCl2 Escherichia coli ADP + UDP
-
 r
2.7.4.22 GTP + UMP reaction rate is 3-5% of that with ATP Escherichia coli GDP + UDP
-
 r
2.7.4.22 additional information no reaction with dUMP, CMP, dCMP or TMP as substrate Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 hexamer alpha6, sedimentation equilibrium Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP-kinase
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.22 51
-
 mutant D201N is half-inactivated Escherichia coli
2.7.4.22 64
-
 wild-type protein is half-inactivated Escherichia coli