EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.3 | industry | the enzyme is useful in industrial applications as glycoside hydrolase | Rhizopus arrhizus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.3 | W47A | site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme | Rhizopus arrhizus |
3.2.1.3 | Y32A | site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme | Rhizopus arrhizus |
3.2.1.3 | Y32A/Y47A | site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme | Rhizopus arrhizus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | additional information | - |
additional information | ligand binding kinetics, wild-type enzyme and mutants | Rhizopus arrhizus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | starch + H2O | Rhizopus arrhizus | - |
starch + beta-D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Rhizopus arrhizus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.3 | (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose | structure-function relationship, ligand binding | Rhizopus arrhizus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | alpha-cyclodextrin + H2O | - |
Rhizopus arrhizus | alpha-cyclodextrin + beta-D-glucose | - |
? | |
3.2.1.3 | beta-cyclodextrin + H2O | - |
Rhizopus arrhizus | beta-cyclodextrin + beta-D-glucose | - |
? | |
3.2.1.3 | gamma-cyclodextrin + H2O | - |
Rhizopus arrhizus | gamma-cyclodextrin + beta-D-glucose | - |
? | |
3.2.1.3 | isomaltotriose + H2O | - |
Rhizopus arrhizus | ? + beta-D-glucose | - |
? | |
3.2.1.3 | maltoheptaose + H2O | - |
Rhizopus arrhizus | maltohexaose + D-glucose | - |
? | |
3.2.1.3 | maltohexaose + H2O | - |
Rhizopus arrhizus | maltopentaose + D-glucose | - |
? | |
3.2.1.3 | maltopentaose + H2O | - |
Rhizopus arrhizus | maltotetraose + beta-D-glucose | - |
? | |
3.2.1.3 | maltotriose + H2O | - |
Rhizopus arrhizus | maltose + beta-D-glucose | - |
? | |
3.2.1.3 | starch + H2O | - |
Rhizopus arrhizus | starch + beta-D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.3 | More | secondary structure analysis, the enzyme contains a family 21 carbohydrate-binding module involving residues W47, Y83, and Y93, the enzyme consists of a catalytic domain and a starch binding domain connected by an O-glycosylated peptide linker located at the N-terminus, molecular modelling, structure-function relatioship | Rhizopus arrhizus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.3 | GA | - |
Rhizopus arrhizus |
3.2.1.3 | glucoamylase | - |
Rhizopus arrhizus |
3.2.1.3 | More | the enzyme belongs to the glycohydrolase family 21 | Rhizopus arrhizus |