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Literature summary extracted from

  • Fassy, F.; Krebs, O.; Lowinski, M.; Ferrari, P.; Winter, J.; Collard-Dutilleul, V.; Salahbey Hocini, K.
    UMP kinase from Streptococcus pneumoniae: evidence for co-operative ATP binding and allosteric regulation (2004), Biochem. J., 384, 619-627.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 GTP increases the affinity for ATP Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22 expression in Escherichia coli BL21 (DE3) Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 GTP at high concentrations Streptococcus pneumoniae
2.7.4.22 UTP decreases the affinity for ATP Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.1
-
 UMP in the presence of MgCl2 Streptococcus pneumoniae
2.7.4.22 0.15
-
 UMP in the presence of MnCl2 Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.22 MgCl2 in the presence of MgCl2 the saturation kinetics of recombinant purified UMP kinase are hyperbolic for UMP and sigmoidal for ATP Streptococcus pneumoniae
2.7.4.22 MnCl2 in the presence of MnCl2 the saturation kinetics of recombinant purified UMP kinase are hyperbolic for UMP and sigmoidal for ATP Streptococcus pneumoniae
2.7.4.22 additional information UMP kinase is totally inactive in the absence of MgCl2 or MnCl2 Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 26300
-
 mass spectroscopy, N-terminal sequencing Streptococcus pneumoniae
2.7.4.22 87500
-
 minor form, gel filtration Streptococcus pneumoniae
2.7.4.22 189000
-
 major form, representing two-thirds of the protein population, gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Streptococcus pneumoniae
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Streptococcus pneumoniae Q97R83
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22 the recombinant enzyme from Escherichia coli Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 additional information
-
 GTP and UTP are extremely poor substrates with an activity below 1 micromol/min/mg with MnCl2 or MgCl2 Streptococcus pneumoniae
2.7.4.22 66
-
 with MgCl2 and ATP as phosphate donor Streptococcus pneumoniae
2.7.4.22 130
-
 with MnCl2 and ATP as phosphate donor Streptococcus pneumoniae
2.7.4.22 135
-
 at pH 6.5, in the presence of MnCl2 Streptococcus pneumoniae
2.7.4.22 182
-
 at pH 6.5, in the presence of MgCl2 Streptococcus pneumoniae
2.7.4.22 274
-
 at pH 7.5, in the presence of MnCl2 Streptococcus pneumoniae
2.7.4.22 399
-
 at pH 7.5, in the presence of MgCl2 Streptococcus pneumoniae
2.7.4.22 666
-
 at pH 8.5, in the presence of MnCl2 Streptococcus pneumoniae

Storage Stability

EC Number Storage Stability Organism
2.7.4.22 -80°C, 12 months storage, less than 10% loss of activity Streptococcus pneumoniae
2.7.4.22 0°C, 6 h, less than 10% loss of activity Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Streptococcus pneumoniae ADP + UDP
-
 ?
2.7.4.22 ATP + UMP at 37°C, pH 7.5, in the presence of MnCl2 or MgCl2 Streptococcus pneumoniae ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 More calculations indicate a heptamer of the major form and a trimer of the minor one. Homoheptamers are not frequently observed and the major form may, rather, be a hexamer Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP kinase
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.22 120
-
 ATP in the presence of MnCl2 Streptococcus pneumoniae
2.7.4.22 175
-
 ATP in the presence of MgCl2 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.22 additional information
-
 below 6.5 with 2 mM ATP, in the presence of MgCl2 Streptococcus pneumoniae
2.7.4.22 additional information
-
 below 6.5 with 2 mM ATP, in the presence of MnCl2 Streptococcus pneumoniae
2.7.4.22 6.5
-
 with 4 mM ATP, in the presence of MgCl2 Streptococcus pneumoniae
2.7.4.22 7.1
-
 with 4 mM ATP, in the presence of MnCl2 Streptococcus pneumoniae
2.7.4.22 7.4
-
 with 10 mM ATP, in the presence of MgCl2 Streptococcus pneumoniae
2.7.4.22 7.8
-
 with 10 mM ATP, in the presence of MnCl2 Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.22 0.14
-
 GTP in the presence of MnCl2 Streptococcus pneumoniae
2.7.4.22 0.21
-
 GTP in the presence of MgCl2 Streptococcus pneumoniae
2.7.4.22 0.21
-
 UTP in the presence of MnCl2 Streptococcus pneumoniae
2.7.4.22 0.28
-
 UTP in the presence of MgCl2 Streptococcus pneumoniae