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Literature summary extracted from

  • Wang, J.Y.; Chao, Y.P.
    Immobilization of cells with surface-displayed chitin-binding domain (2006), Appl. Environ. Microbiol., 72, 927931.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.14 molecular biology recombinant Lpp-OmpA-ChBD fusion protein is useful for stable and highly specific immobilization of cells, displaying the fusion protein on the cell surface, on chitinous material, method optimization, overview Niallia circulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 the chitin-binding domain CBD of the enzyme is fused to genes lpp and ompA and the Lpp-OmpA-ChBD hybrid is expressed in Escherichia coli strain BL21(DE3) directed to the cell surface Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Niallia circulans
-
 ?
-
 ?
3.2.1.14 chitin + H2O Niallia circulans WL-12
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Niallia circulans
-
-
-
3.2.1.14 Niallia circulans WL-12
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.14 chitin + H2O the chitin-binding domain CBD exhibits high specificity for and stable binding to chitin at pH 5.0-8.0 and 15-37°C Niallia circulans ?
-
 ?
3.2.1.14 chitin + H2O
-
 Niallia circulans WL-12 ?
-
 ?
3.2.1.14 chitin + H2O the chitin-binding domain CBD exhibits high specificity for and stable binding to chitin at pH 5.0-8.0 and 15-37°C Niallia circulans WL-12 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 More the chitin-binding domain ChBD comprises 45 amino acid residues, a tightly packed structure, and exhibits high specificity for and stable binding to chitin Niallia circulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 chitinase A1
-
 Niallia circulans