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Literature summary extracted from

  • Kim, M.S.; Park, J.T.; Kim, Y.W.; Lee, H.S.; Nyawira, R.; Shin, H.S.; Park, C.S.; Yoo, S.H.; Kim, Y.R.; Moon, T.W.; Park, K.H.
    Properties of a novel thermostable glucoamylase from the hyperthermophilic archaeon Sulfolobus solfataricus in relation to starch processing (2004), Appl. Environ. Microbiol., 70, 3933-3940.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.3 biotechnology the enzyme is potentially useful in improvement of industrial starch processing by eliminating the need to adjust both pH and temperature Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 gene ssg, phylogenetic analysis, expression in Escherichia coli Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Saccharolobus solfataricus Q97ZD0 gene ssg
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 recombinant enzyme 80.9fold from Escherichia coli by heat treatment at 70°C, hydrophobic interaction chromatography, and gel filtration Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.3 (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose reaction mechanism depends on the substrate, i.e. hydrolysis or transglycosylation, overview Saccharolobus solfataricus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 56.3
-
 purified recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 maltooligomers + H2O substrate specificity Saccharolobus solfataricus ? + beta-D-glucose
-
 r
3.2.1.3 maltotriose + H2O exoglycosidic hydrolysis of terminal glucose in forward reaction, preferred substrate, synthesis of isomaltose from beta-D-glucose in the reverse reaction with low activity Saccharolobus solfataricus maltose + beta-D-glucose anomeric product configuration analysis at 80°C r
3.2.1.3 additional information bifunctional enzyme performing hydrolysis or transglycosylation depending on the substrate Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 tetramer
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Saccharolobus solfataricus
3.2.1.3 SSG
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 90
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 60 100 90% of maximal activity at 80°C, low activity at 100°C Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information substrate specificity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 4 8
-
 Saccharolobus solfataricus