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Literature summary extracted from

  • Huston, A.L.; Methe, B.; Deming, J.W.
    Purification, characterization, and sequencing of an extracellular cold-active aminopeptidase produced by marine psychrophile Colwellia psychrerythraea strain 34H (2004), Appl. Environ. Microbiol., 70, 3321-3328.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.6 additional information the enzyme is cold-active Colwellia psychrerythraea
3.4.11.22 additional information the enzyme is cold-active Colwellia psychrerythraea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 DNA and amino acid sequence determination and analysis, phylogenetic analysis Colwellia psychrerythraea
3.4.11.22 DNA and amino acid sequence determination and analysis, phylogenetic analysis Colwellia psychrerythraea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 Ca2+ 16% inhibition at 1 mM, 24% inhibition at 10 mM Colwellia psychrerythraea
3.4.11.6 DTT 25% inhibition at 1 mM, complete inhibition at 10 mM Colwellia psychrerythraea
3.4.11.6 EDTA complete inhibition at 1-10 mM Colwellia psychrerythraea
3.4.11.6 Mn2+ complete inhibition at 10 mM Colwellia psychrerythraea
3.4.11.6 Zn2+ complete inhibition at 0.1-10 mM Colwellia psychrerythraea
3.4.11.22 Ca2+ 16% inhibition at 1 mM, 24% inhibition at 10 mM Colwellia psychrerythraea
3.4.11.22 DTT 25% inhibition at 1 mM, complete inhibition at 10 mM Colwellia psychrerythraea
3.4.11.22 EDTA complete inhibition at 1-10 mM Colwellia psychrerythraea
3.4.11.22 Mn2+ complete inhibition at 10 mM Colwellia psychrerythraea
3.4.11.22 Zn2+ complete inhibition at 0.1-10 mM Colwellia psychrerythraea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.043
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, -1°C Colwellia psychrerythraea
3.4.11.6 0.058
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 9°C Colwellia psychrerythraea
3.4.11.6 0.072
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 19°C Colwellia psychrerythraea
3.4.11.22 0.043
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, -1°C Colwellia psychrerythraea
3.4.11.22 0.058
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 9°C Colwellia psychrerythraea
3.4.11.22 0.072
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 19°C Colwellia psychrerythraea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 extracellular
-
 Colwellia psychrerythraea
-
-
3.4.11.22 extracellular
-
 Colwellia psychrerythraea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Mg2+ 22% activation at 10 Colwellia psychrerythraea
3.4.11.6 NaCl absolutely required for activity Colwellia psychrerythraea
3.4.11.22 Mg2+ 22% activation at 10 Colwellia psychrerythraea
3.4.11.22 NaCl absolutely required for activity Colwellia psychrerythraea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 71000
-
 gel filtration Colwellia psychrerythraea
3.4.11.22 71000
-
 gel filtration Colwellia psychrerythraea

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Colwellia psychrerythraea
-
 marine psychrophile, strain 34H
-
3.4.11.22 Colwellia psychrerythraea
-
 marine psychrophile, strain 34H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 native enzyme 460fold to homogeneity by ion exchange and hydroxy apatite chromatography, and ion exchange chromatography Colwellia psychrerythraea
3.4.11.22 native enzyme 460fold to homogeneity by ion exchange and hydroxy apatite chromatography, and ion exchange chromatography Colwellia psychrerythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 azocasein + H2O
-
 Colwellia psychrerythraea protein fragments of azocasein of MW 71 kDa, 83 kDa, and 22 kDa
-
 ?
3.4.11.6 L-Ala-7-amido-4-methylcoumarin + H2O best substrate Colwellia psychrerythraea L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
-
 Colwellia psychrerythraea L-Ala-Ala-Pro-Leu + 4-nitroaniline
-
 ?
3.4.11.6 L-Arg-7-amido-4-methylcoumarin + H2O high activity Colwellia psychrerythraea L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Colwellia psychrerythraea L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 additional information substrate specificity, no activity with L-Pro-7-amido-4-methylcoumarin and L-Asp-7-amido-4-methylcoumarin, L-Ser-7-amido-4-methylcoumarin is a poor substrate, overview Colwellia psychrerythraea ?
-
 ?
3.4.11.22 azocasein + H2O
-
 Colwellia psychrerythraea protein fragments of azocasein of MW 71 kDa, 83 kDa, and 22 kDa
-
 ?
3.4.11.22 L-Ala-7-amido-4-methylcoumarin + H2O best substrate Colwellia psychrerythraea L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
-
 Colwellia psychrerythraea L-Ala-Ala-Pro-Leu + 4-nitroaniline
-
 ?
3.4.11.22 L-Arg-7-amido-4-methylcoumarin + H2O high activity Colwellia psychrerythraea L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 L-Leu-7-amido-4-methylcoumarin + H2O
-
 Colwellia psychrerythraea L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.22 additional information substrate specificity, no activity with L-Pro-7-amido-4-methylcoumarin and L-Asp-7-amido-4-methylcoumarin, L-Ser-7-amido-4-methylcoumarin is a poor substrate, overview Colwellia psychrerythraea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.6 monomer 1 * 71000, SDS-PAGE Colwellia psychrerythraea
3.4.11.22 monomer 1 * 71000, SDS-PAGE Colwellia psychrerythraea

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 ColAP
-
 Colwellia psychrerythraea
3.4.11.6 cold-active aminopeptidase
-
 Colwellia psychrerythraea
3.4.11.6 LAPase
-
 Colwellia psychrerythraea
3.4.11.6 More the enzyme belongs to the M1 peptidase family Colwellia psychrerythraea
3.4.11.22 ColAP
-
 Colwellia psychrerythraea
3.4.11.22 cold-active aminopeptidase
-
 Colwellia psychrerythraea
3.4.11.22 LAPase
-
 Colwellia psychrerythraea
3.4.11.22 More the enzyme belongs to the M1 peptidase family Colwellia psychrerythraea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.6 additional information
-
 the enzyme is cold-active Colwellia psychrerythraea
3.4.11.6 20
-
 assay at Colwellia psychrerythraea
3.4.11.22 additional information
-
 the enzyme is cold-active Colwellia psychrerythraea
3.4.11.22 20
-
 assay at Colwellia psychrerythraea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.6 30
-
 half-life is 67 min Colwellia psychrerythraea
3.4.11.6 40
-
 half-life is 38 min Colwellia psychrerythraea
3.4.11.6 45
-
 half-life is 10 min Colwellia psychrerythraea
3.4.11.6 50
-
 half-life is 5 min Colwellia psychrerythraea
3.4.11.22 30
-
 half-life is 67 min Colwellia psychrerythraea
3.4.11.22 40
-
 half-life is 38 min Colwellia psychrerythraea
3.4.11.22 45
-
 half-life is 10 min Colwellia psychrerythraea
3.4.11.22 50
-
 half-life is 5 min Colwellia psychrerythraea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.6 0.043
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, -1°C Colwellia psychrerythraea
3.4.11.6 0.13
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 9°C Colwellia psychrerythraea
3.4.11.6 0.36
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 19°C Colwellia psychrerythraea
3.4.11.22 0.043
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, -1°C Colwellia psychrerythraea
3.4.11.22 0.13
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 9°C Colwellia psychrerythraea
3.4.11.22 0.36
-
 L-Ala-Ala-Pro-Leu-4-nitroanilide pH 7.2, 19°C Colwellia psychrerythraea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 7.2
-
 assay at Colwellia psychrerythraea
3.4.11.22 7.2
-
 assay at Colwellia psychrerythraea