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Literature summary extracted from
- Identification of quorum-quenching N-acyl homoserine lactonases from Bacillus species (2002), Appl. Environ. Microbiol., 68, 1754-1759.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.81 | gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus cereus |
| 3.1.1.81 | gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus mycoides |
| 3.1.1.81 | gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | gene aiiA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Bacillus thuringiensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.81 | D191S | site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant | Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | D236S | site-directed mutagenesis, the mutation does not affect the enzyme activity | Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | H235S | site-directed mutagenesis, the mutation does not affect the enzyme activity | Bacillus sp. (in: Bacteria) |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.81 | Zn2+ | metallohydrolase containing the conserved zinc-binding motif | Bacillus cereus |  |
| 3.1.1.81 | Zn2+ | metallohydrolase containing the conserved zinc-binding motif | Bacillus mycoides | |
| 3.1.1.81 | Zn2+ | metallohydrolase containing the conserved zinc-binding motif | Bacillus thuringiensis | |
| 3.1.1.81 | Zn2+ | metallohydrolase containing the conserved zinc-binding motif, residues H106, H109, D108, and H169 of the strain 240B1 enzyme are required for Zn2+ binding and full enzyme activity, overview | Bacillus sp. (in: Bacteria) | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Bacillus cereus | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Bacillus mycoides | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Bacillus sp. (in: Bacteria) | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Bacillus thuringiensis | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | N-acyl-(S)-homoserine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.81 | Bacillus cereus | - |
- |
- |
| 3.1.1.81 | Bacillus mycoides | - |
- |
- |
| 3.1.1.81 | Bacillus sp. (in: Bacteria) | Q8RPW9 | gene aiiA from Bacillus sp. strain COT1; strains 240B1 and COT1 | - |
| 3.1.1.81 | Bacillus thuringiensis | A3FJ64 | aiiA from strain B1; several subspecies and strains, overview | - |
| 3.1.1.81 | Bacillus thuringiensis | A3FJ64 | aiiA from strain B2; several subspecies and strains, overview | - |
| 3.1.1.81 | Bacillus thuringiensis | Q8RJA0 | aiiA from strain B20; several subspecies and strains, overview | - |
| 3.1.1.81 | Bacillus thuringiensis | Q8RPW5 | aiiA from strain B18; several subspecies and strains, overview | - |
| 3.1.1.81 | Bacillus thuringiensis | Q8RPW6 | aiiA from strain B17; several subspecies and strains, overview | - |
| 3.1.1.81 | no activity in Bacillus fusiformis | - |
- |
- |
| 3.1.1.81 | no activity in Bacillus sphaericus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus cereus | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus mycoides | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus sp. (in: Bacteria) | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants | Bacillus thuringiensis | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus cereus | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus mycoides | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus sp. (in: Bacteria) | N-acyl-(S)-homoserine | - |
? | |
| 3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the acylhomoserine lactones are inducers of quorum-sensing signaling | Bacillus thuringiensis | N-acyl-(S)-homoserine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.81 | AHL lactonase | - |
Bacillus cereus |
| 3.1.1.81 | AHL lactonase | - |
Bacillus mycoides |
| 3.1.1.81 | AHL lactonase | - |
Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | AHL lactonase | - |
Bacillus thuringiensis |
| 3.1.1.81 | AHL-inactivating enzyme | - |
Bacillus cereus |
| 3.1.1.81 | AHL-inactivating enzyme | - |
Bacillus mycoides |
| 3.1.1.81 | AHL-inactivating enzyme | - |
Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | AHL-inactivating enzyme | - |
Bacillus thuringiensis |
| 3.1.1.81 | AHL-lactonase | - |
Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | AHL-lactonase | - |
Bacillus thuringiensis |
| 3.1.1.81 | quorum-quenching N-acyl homoserine lactonase | - |
Bacillus cereus |
| 3.1.1.81 | quorum-quenching N-acyl homoserine lactonase | - |
Bacillus mycoides |
| 3.1.1.81 | quorum-quenching N-acyl homoserine lactonase | - |
Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | quorum-quenching N-acyl homoserine lactonase | - |
Bacillus thuringiensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.81 | 28 | - |
in vivo assay at | Bacillus cereus |
| 3.1.1.81 | 28 | - |
in vivo assay at | Bacillus mycoides |
| 3.1.1.81 | 28 | - |
in vivo assay at | Bacillus sp. (in: Bacteria) |
| 3.1.1.81 | 28 | - |
in vivo assay at | Bacillus thuringiensis |
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