Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gacar, G.G.; Midilli, K.; Kolayli, F.; Ergen, K.; Gundes, S.; Hosoglu, S.; Karadenizli, A.; Vahaboglu, H.
    Genetic and enzymatic properties of metallo-beta-lactamase VIM-5 from a clinical isolate of Enterobacter cloacae (2005), Antimicrob. Agents Chemother., 49, 4400-4403.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 EDTA 0.02 mM, 50% inhibition Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.012
-
 Imipenem
-
 Enterobacter cloacae
3.5.2.6 0.049
-
 meropenem
-
 Enterobacter cloacae
3.5.2.6 0.076
-
 cefepime
-
 Enterobacter cloacae
3.5.2.6 0.101
-
 cefotaxime
-
 Enterobacter cloacae
3.5.2.6 0.113
-
 penicillin
-
 Enterobacter cloacae
3.5.2.6 0.125
-
 ampicillin
-
 Enterobacter cloacae
3.5.2.6 0.149
-
 ceftazidime
-
 Enterobacter cloacae
3.5.2.6 1.753
-
 piperacillin
-
 Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 28000
-
 x * 28000, SDS-PAGE Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Enterobacter cloacae Q4ZE97
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 26
-
-
 Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 a penicillin + H2O
-
 Enterobacter cloacae a penicilloic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefepime + H2O
-
 Enterobacter cloacae (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Enterobacter cloacae (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Enterobacter cloacae (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 imipenem + H2O
-
 Enterobacter cloacae (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Enterobacter cloacae (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 penicillin + H2O
-
 Enterobacter cloacae ?
-
 ?
3.5.2.6 piperacillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 ? x * 28000, SDS-PAGE Enterobacter cloacae

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 metallo-beta-lactamase
-
 Enterobacter cloacae
3.5.2.6 VIM-5
-
 Enterobacter cloacae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.1
-
 cefepime
-
 Enterobacter cloacae
3.5.2.6 0.2
-
 ceftazidime
-
 Enterobacter cloacae
3.5.2.6 2.4
-
 meropenem
-
 Enterobacter cloacae
3.5.2.6 3.5
-
 Imipenem
-
 Enterobacter cloacae
3.5.2.6 9.2
-
 cefotaxime
-
 Enterobacter cloacae
3.5.2.6 14
-
 ampicillin
-
 Enterobacter cloacae
3.5.2.6 29
-
 penicillin
-
 Enterobacter cloacae
3.5.2.6 47
-
 piperacillin
-
 Enterobacter cloacae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Enterobacter cloacae isoelectric focusing
-
 4.5