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Literature summary extracted from

  • Girlich, D.; Naas, T.; Nordmann, P.
    Biochemical characterization of the naturally occurring oxacillinase OXA-50 of Pseudomonas aeruginosa (2004), Antimicrob. Agents Chemother., 48, 2043-2048.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 cloned into a multicopy plasmid and expressed in Escherichia coli and Pseudomonas aeruginosa Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.02
-
 Imipenem 30°C, pH 7.0 Pseudomonas aeruginosa
3.5.2.6 0.2
-
 nitrocefin 30°C, pH 7.0 Pseudomonas aeruginosa
3.5.2.6 0.8
-
 benzylpenicillin 30°C, pH 7.0 Pseudomonas aeruginosa
3.5.2.6 0.9
-
 cephalotin 30°C, pH 7.0 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 25000
-
 x * 25000, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pseudomonas aeruginosa Q6PL88
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 75.5
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefsulodin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(4-carbamoylpyridin-1-ium-1-yl)methyl]-2-[(R)-carboxy{[(2R)-2-phenyl-2-sulfoacetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalotin + H2O
-
 Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Pseudomonas aeruginosa (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Pseudomonas aeruginosa (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ticarcillin + H2O
-
 Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 ? x * 25000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 Oxa-50
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.1
-
 Imipenem 30°C, pH 7.0 Pseudomonas aeruginosa
3.5.2.6 0.2
-
 cephalotin 30°C, pH 7.0 Pseudomonas aeruginosa
3.5.2.6 100
-
 nitrocefin 30°C, pH 7.0 Pseudomonas aeruginosa
3.5.2.6 110
-
 benzylpenicillin 30°C, pH 7.0 Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Pseudomonas aeruginosa isoelectric focusing
-
 8.6