Any feedback?
Literature summary extracted from
- A fluorogenic substrate for detection of organophosphatase activity (2004), Anal. Biochem., 327, 140-148.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | gene PON1, expression of two naturally occurring allelic variant isozymes L55R192 PON1 and L55Q192 PON1 in Escherichia coli strain DH5alpha and in CHO cells | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | additional information | - |
additional information | kinetics | Homo sapiens | |
| 3.1.8.1 | 0.091 | - |
7-diethylphospho-6,8-difluor-4-methylumbelliferyl | pH 8.0, 37°C, recombinant isozyme L55R192 PON1 | Homo sapiens |  |
| 3.1.8.1 | 0.2 | - |
7-diethylphospho-6,8-difluor-4-methylumbelliferyl | pH 8.0, 37°C, recombinant isozyme L55Q192 PON1 | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.8.1 | CaCl2 | added to optimized fluorescence assay | Homo sapiens | |
| 3.1.8.1 | NaCl | added to optimized fluorescence assay | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.8.1 | Homo sapiens | - |
gene PON1, two naturally occuring allelic variant isozymes L55R192 PON1 and L55Q192 PON1 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.8.1 | liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.1 | 7-diethylphospho-6,8-difluor-4-methylumbelliferyl + H2O | high level of hydrolysis of the fluorogenic substrate, fluorescence assay method optimization | Homo sapiens | diethylphosphate + 6,8-difluor-4-methylumbelliferol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | OPH | - |
Homo sapiens |
| 3.1.8.1 | organophosphorus hydrolase | - |
Homo sapiens |
| 3.1.8.1 | paraoxonase | - |
Homo sapiens |
| 3.1.8.1 | PON | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 8 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
