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Literature summary extracted from

  • Howard, R.L.
    Refolding and characterization of a heterologous expressed Phanerochaete chrysosporium cellobiohydrolase (CBHl.2) (2005), Afr. J. Biotechnol., 4, 1185-1188.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 gene cbhl.2, expression in Escherichia coli strain XL 1-Blue in membranes and inclusion bodies Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Phanerodontia chrysosporium Q7LHI2 i.e. Sporotrichum pruinosum, strain ME446, gene cbhl.2
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.91 refolding of recombinant enzyme, after solubilization and denaturation by 8 M urea from Escherichia coli inclusion bodies, by dialysis of enzyme in 10 mM DTT, PMSF, 2-mercaptoethanol, at 4°C against 1 l of 50 mM Tris, pH 8.0, containing 5 mM CaCl2, for 2 days with 3times of buffer change Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.91 1.6
-
 purified recombinant enzyme, substrate 4-methylumbelliferyl beta-D-lactoside Phanerodontia chrysosporium
3.2.1.91 2.15
-
 purified recombinant enzyme, substrate 4-methylumbelliferyl beta-D-cellobioside Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Phanerodontia chrysosporium 4-methylumbelliferol + beta-D-cellobiose
-
 ?
3.2.1.91 4-methylumbelliferyl beta-D-lactoside + H2O
-
 Phanerodontia chrysosporium 4-methylumbelliferol + beta-D-lactose
-
 ?
3.2.1.91 4-nitrophenyl beta-cellobioside + H2O
-
 Phanerodontia chrysosporium 4-nitrophenol + beta-cellobiose
-
 ?
3.2.1.91 4-nitrophenyl beta-lactoside + H2O
-
 Phanerodontia chrysosporium 4-nitrophenol + beta-lactose
-
 ?
3.2.1.91 avicel + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
3.2.1.91 cellulose + H2O the enzyme hydrolyzes beta-1,4-linkages Phanerodontia chrysosporium cellobiose + ?
-
 ?
3.2.1.91 additional information substrate specificity, overview Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 50000-60000, recombinant enzyme, SDS-PAGE Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 Cbhl.2
-
 Phanerodontia chrysosporium
3.2.1.91 cellobiohydrolase
-
 Phanerodontia chrysosporium
3.2.1.91 Cellulase
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 50
-
 assay at Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 100
-
 5 min, loss of 50% of activity of the purified recombinant enzyme Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 5
-
 assay at Phanerodontia chrysosporium