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Literature summary extracted from

  • Yamasaki, M.; Ogura, K.; Moriwaki, S.; Hashimoto, W.; Murata, K.; Mikami, B.
    Crystallization and preliminary X-ray analysis of alginate lyases A1-II and A1-II' from Sphingomonas sp. A1 (2005), Acta Crystallogr. Sect. F, 61, 288-290.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.11 expression in Escherichia coli Sphingomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.3 recombinant, alginate lyase A1-II and alginate lyase A1-II'. Both proteins are crystallized at 20°C using hanging-drop vapour-diffusion method. A crystal of A-1II belongs to space group P2(1) and diffracts to 2.2 A resolution, with unit-cell parameters a = 51.3 A, b = 30.1 A, c = 101.6 A, beta = 100.2°. Crystals of A1-II' belong to space group P2(1)2(1)2(1) and diffract to 1.0 A resolution, with unit-cell parameters a = 34.6, b = 68.5, c = 80.3 A Sphingomonas sp.
4.2.2.11 hanging-drop vapour-diffusion, A1-II structure at 2.2 A resolution, A1-II' structure at 1.0 A resolution Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.11 25000
-
 A1-II, SDS-PAGE Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Sphingomonas sp.
-
 A1
-
4.2.2.11 Sphingomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant Sphingomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase A1-II
-
 Sphingomonas sp.
4.2.2.3 alginate lyase A1-II'
-
 Sphingomonas sp.
4.2.2.11 alginate lyase A1-II and its homologue A1-II' Sphingomonas sp.