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Literature summary extracted from

  • Bagautdinov, B.; Kuroishi, C.; Sugahara, M.; Kunishima, N.
    Purification, crystallization and preliminary crystallographic analysis of the biotin-protein ligase from Pyrococcus horikoshii OT3 (2005), Acta Crystallogr. Sect. F, 61, 193-195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21 Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.15 purified recombinant native and selenomethionine-labeled enzyme, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.15 26071
-
 2 * 26071 recombinant enzyme, SDS-PAGE and amino acid sequence calculation Pyrococcus horikoshii
6.3.4.15 53900
-
 recombinant enzyme, light scattering study Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Pyrococcus horikoshii
-
 strain OT3
-
6.3.4.15 Pyrococcus horikoshii OT-3
-
 strain OT3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 recombinant enzyme from Escherichia coli strain BL21 in a chromatographic procedure comprising several steps Pyrococcus horikoshii

Subunits

EC Number Subunits Comment Organism
6.3.4.15 dimer 2 * 26071 recombinant enzyme, SDS-PAGE and amino acid sequence calculation Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin-protein ligase
-
 Pyrococcus horikoshii