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Literature summary extracted from

  • Nureki, O.; Watanabe, K.; Fukai, S.; Ishii, R.; Endo, Y.; Hori, H.; Yokoyama, S.
    Deep knot structure for construction of active site and cofactor binding site of tRNA modification enzyme (2004), Structure, 12, 593-602.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.34 in complex with S-adenosyl-L-methionine Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.34 E124A 13fold increase in Km-value Thermus thermophilus
2.1.1.34 I142A kinetic parameters comparable to wild-type Thermus thermophilus
2.1.1.34 L101A 13fold increase in Km-value Thermus thermophilus
2.1.1.34 L151A 5fold increase in Km-value Thermus thermophilus
2.1.1.34 M144A 15fold increase in Km-value Thermus thermophilus
2.1.1.34 N152A 80fold increase in Km-value Thermus thermophilus
2.1.1.34 R41A no enzymic activity Thermus thermophilus
2.1.1.34 R41K 30fold increase in Km-value Thermus thermophilus
2.1.1.34 S150A large increase in Km-value Thermus thermophilus
2.1.1.34 T99A small increase in Km-value Thermus thermophilus
2.1.1.34 V128A small increase in Km-value Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.34 0.01
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant I142A Thermus thermophilus
2.1.1.34 0.01
-
 S-adenosyl-L-methionine pH 7.6, 50°C, wild-type Thermus thermophilus
2.1.1.34 0.014
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant V128A Thermus thermophilus
2.1.1.34 0.017
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant T99A Thermus thermophilus
2.1.1.34 0.056
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant L151A Thermus thermophilus
2.1.1.34 0.13
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant L101A Thermus thermophilus
2.1.1.34 0.15
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant M144A Thermus thermophilus
2.1.1.34 0.3
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant R41K Thermus thermophilus
2.1.1.34 0.8
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant N152A Thermus thermophilus
2.1.1.34 1.7
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant S150A Thermus thermophilus
2.1.1.34 28
-
 S-adenosyl-L-methionine pH 7.6, 50°C, mutant E124A Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.34 Thermus thermophilus Q9FAC4
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA mechanism, upon binding of guanine 18, a RNA main chain phosphate group activates R41, which then deprotonates the 2'-OH-group for methylation Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.34 S-adenosyl-L-methionine + tRNA
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
-
 ?