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Literature summary extracted from

  • Keatinge-Clay, A.T.; Shelat, A.A.; Savage, D.F.; Tsai, S.C.; Miercke, L.J.; O'Connell, J.D., 3rd; Khosla, C.; Stroud, R.M.
    Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase (2003), Structure, 11, 147-154.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 gene fabD, expression of His-tagged enzyme in Escherichia coli strain BL21 Streptomyces coelicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.39 recombinant purified enzyme, 3 mg/ml protein in 10 mM Tris-HCl, pH 7.4, 1 mM 2-mercaptoethanol, hanging drop vapoir diffusion method, 3 days, room temperature, soaking of crystals in 30% PEG 4000, 100 mM sodium acetate, pH 4.8, 200 mM ammonium acetate, and 20% glycerol for cryoprotection, X-ray diffraction structure determination and analysis at 2.0 A resolution, macromolecular docking simulation with K47A/K190A/R287A/K293A mutant actinorhodin ACP structure, overview Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + [acyl-carrier protein] Streptomyces coelicolor enzyme is involved in both fatty acid and polyketide synthesis pathways CoA + malonyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Streptomyces coelicolor
-
 gene fabD
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity Streptomyces coelicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] catalytic mechanism, [acyl-carrier protein] binding site, residues Phe200 and Met126 are involved Streptomyces coelicolor
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + [acyl-carrier protein]
-
 Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + [acyl-carrier protein] enzyme is involved in both fatty acid and polyketide synthesis pathways Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 malonyl-CoA:ACP transacylase
-
 Streptomyces coelicolor
2.3.1.39 MAT
-
 Streptomyces coelicolor