Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bellinzoni, M.; Buroni, S.; Pasca, M.R.; Guglierame, P.; Arcesi, F.; De Rossi, E.; Riccardi, G.
    Glutamine amidotransferase activity of NAD+ synthetase from Mycobacterium tuberculosis depends on an amino-terminal nitrilase domain (2005), Res. Microbiol., 156, 173-177.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.1
-
 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.1 C176A complete loss of glutamine-dependent activity, retains 90% of its NH3-dependent activity Mycobacterium tuberculosis
6.3.5.1 E52A complete loss of glutamine-dependent activity, retains 30% of its NH3-dependent activity Mycobacterium tuberculosis
6.3.5.1 K121A complete loss of glutamine-dependent and NH3-dependent activity Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.1 0.5
-
 deamido-NAD+ wild-type enzyme Mycobacterium tuberculosis
6.3.5.1 0.8
-
 ATP wild-type enzyme Mycobacterium tuberculosis
6.3.5.1 0.8
-
 ATP mutant enzyme E52A Mycobacterium tuberculosis
6.3.5.1 1.3
-
 ATP mutant enzyme C176A Mycobacterium tuberculosis
6.3.5.1 1.4
-
 deamido-NAD+ mutant enzyme C176A Mycobacterium tuberculosis
6.3.5.1 1.4
-
 deamido-NAD+ mutant enzyme E52A Mycobacterium tuberculosis
6.3.5.1 1.6
-
 L-Gln wild-type enzyme Mycobacterium tuberculosis
6.3.5.1 1.6
-
 NH4+ mutant enzyme E52A Mycobacterium tuberculosis
6.3.5.1 2.3
-
 NH4+ mutant enzyme C176A Mycobacterium tuberculosis
6.3.5.1 2.7
-
 NH4+ wild-type enzyme Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.1 mutant enzymes expressed in Escherichia coli Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.1 ATP + deamido-NAD+ + L-Gln
-
 Mycobacterium tuberculosis AMP + diphosphate + NAD+ + L-Glu
-
 ?
6.3.5.1 ATP + deamido-NAD+ + NH4+
-
 Mycobacterium tuberculosis AMP + diphosphate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.1 NAD+ synthetase
-
 Mycobacterium tuberculosis