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Literature summary extracted from

  • Chen, S.; Shin, D.H.; Pufan, R.; Kim, R.; Kim, S.H.
    Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2.ANG. resolution (2004), Proteins, 56, 839-843.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.2 DNA sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Mycoplasma pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.3.2 purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM EDTA, 1 mM DTT, at room temperature, 30 mg/ml selenomethionine-labeled enzyme in 0.2 M ammonium sulfate, 0.1 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, at 20°C, flash freezing of all crystals in a solution containing 0.17 M ammonium sulfate, 0.085 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, X-ray diffraction structure determination and analysis at 2.2 A resolution Mycoplasma pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate Mycoplasma pneumoniae
-
 ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Mycoplasma pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to homogeneity Mycoplasma pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.3.2 ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate active site and substrate binding site structure Mycoplasma pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Mycoplasma pneumoniae ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.2 5,10-Methenyltetrahydrofolate synthetase
-
 Mycoplasma pneumoniae
6.3.3.2 Methenyltetrahydrofolate synthetase
-
 Mycoplasma pneumoniae
6.3.3.2 MTHFS
-
 Mycoplasma pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.3.2 ATP
-
 Mycoplasma pneumoniae